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Cyclopentenone Prostaglandin HPLC Mixture

Cayman Chemical Item Number 10000

Cyclopentenone PG HPLC Mixture

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Description

This mixture contains all of the major UV-absorbing cyclopentenone prostaglandins and their precursors. Contents: Prostaglandin A2, Prostaglandin B2, Prostaglandin D2, Prostaglandin E2, Prostaglandin J2, and 15-deoxy-Δ12,14-Prostaglandin J2 (100 µg each). Prostaglandin E2 and Prostaglandin D2 are both chemically unstable. In the absence of any enzymatic influence, both are undergo dehydration to form the highly UV active cyclopentenone compounds PGA2 and PGJ2, respectively.1,2 These compounds absorb in the ultraviolet with maxima at 216 - 220 lambda. The cyclopentenone prostaglandins have been noted to have antimitotic, antiviral and antitumor activity in a number of systems.3,4 PGA2 and PGJ2, can also undergo further elimination and double bond rearrangements to give PGB2 and 15-deoxy-Δ12,14 PGJ2, respectively. 15-deoxy-Δ12,14 PGJ2 is a relatively potent ligand for the PPARγ nuclear receptor. PGB2 does not appear to have significant intrinsic biological activity; however, it is frequently used as a reliable internal standard for analysis of eicosanoids by HPLC.

1 Granström, E., and Samuelsson, B. Quantitative measurement of prostaglandins and thromboxanes: General considerations. Adv Prostaglandin Thromboxane Res 5 1-13 (1978).

2 Fitzpatrick, F.A., and Wynalda, M.A. Albumin-catalyzed metabolism of prostaglandin D2. Identification of products formed in vitro. J Biol Chem 258 11713-11718 (1983).

3 Fukushima, M., Kato, T., Narumiya, S., et al. Prostaglandin A and J: Antitumor and antiviral prostaglandins. Adv Prostaglandin Thromboxane Leukot Res 19 415-418 (1989).

4 Fukushima, M. Prostaglandin J2 - antitumor and anti-viral activities and the mechanisms involved. Eicosanoids 3 189-199 (1990).

Synonyms
  • Cyclopentenone PG HPLC Mixture
Formulation A solution in methyl acetate
Purity ≥98% for each compound
Stability 6 months
Storage -80°C
Shipping DRY ICE in continental US; may vary elsewhere

Background Reading

Salmon, J.A., and Flower, R.J. Extraction and thin-layer chromatography of arachidonic acid metabolites. Methods Enzymol 86 477-493 (1982).

Salari, H., Yeung, M., Douglas, S., et al. Detection of prostaglandins by high-performance liquid chromatography after conversion to p-(9-anthroyloxy)phenacyl esters. Anal Biochem 165 220-229 (1987).

Fukushima, M. Prostaglandin J2 - antitumor and anti-viral activities and the mechanisms involved. Eicosanoids 3 189-199 (1990).

Fitzpatrick, F.A., and Wynalda, M.A. Albumin-catalyzed metabolism of prostaglandin D2. Identification of products formed in vitro. J Biol Chem 258 11713-11718 (1983).

Granström, E., and Samuelsson, B. Quantitative measurement of prostaglandins and thromboxanes: General considerations. Adv Prostaglandin Thromboxane Res 5 1-13 (1978).

Kliewer, S.A., Lenhard, J.M., Willson, T.M., et al. A prostaglandin J2 metabolite binds peroxisome proliferator-activated receptor γ and promotes adipocyte differentiation. Cell 83 813-819 (1995).

Powell, W.S. Reversed-phase high-pressure liquid chromatography of arachidonic acid metabolites formed by cyclooxygenase and lipoxygenases. Anal Biochem 148 59-69 (1985).

Fukushima, M., Kato, T., Narumiya, S., et al. Prostaglandin A and J: Antitumor and antiviral prostaglandins. Adv Prostaglandin Thromboxane Leukot Res 19 415-418 (1989).

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This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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Prostaglandin HPLC Mixture
Prostaglandin J2
Prostaglandin Metabolite HPLC Mixture
Vasoactive Eicosanoid HPLC Mixture
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