Antigen: human CD36 amino acids 99-114 (AKENVTQDAEDNTVSF) · Host: rabbit · Application(s): WB · CD36 is a type-B scavenger receptor that is necessary for the formation of foam cells in atherosclerotic lesions.^1,2,3,4 This membrane glycoprotein can internalize fatty acids which activate PPARγ and stimulate further expression of CD36.^5,6 This positive feedback loop combined with the murine CD36 knock-out studies reinforces the importance of CD36 in lipid metabolism. Additionally CD36 is needed for the phagocytosis of P. falciparum infected erythrocytes, retinal pigment epithelial cell photoreceptor fragments, and post-apoptotic monocytes and neutrophils.^7,8,9,10 CD36 expression has been monitored during hematopoietic cell differentiation and may be an indicator of tumor spreading in lymphocytic leukemia.¹¹ This transmembrane glycoprotein is detected by immunoblotting at 88 kDa or 54 kDa depending on the degree of glycosylation of the receptor in your sample. Positive controls include adipose and heart tissue, platelets, and macrophages.

1 Febbraio, M., Abumrad, N.A., Hajjar, D.P., et al. A null mutation in murine CD36 reveals an important role in fatty acid and lipoprotein metabolism. J Biol Chem 274 19055-19062 (1999).

2 Podrez, E.A., Febbraio, M., Sheibani, N., et al. Macrophage scavenger receptor CD36 is the major receptor for LDL modified by monocyte-generated reactive nitrogen species. J Clin Invest 105(8) 1095-1108 (2000).

3 Febbraio, M., Podrez, E.A., Smith, J.D., et al. Targeted disruption of the class B scavenger receptor CD36 protects against atherosclerotic lesion development in mice. J Clin Invest 105(8) 1049-1056 (2000).

4 Febbraio, M., Hajjar, D.P., and Silverstein, R.L. CD36: A class B scavenger receptor involved in angiogenesis, atherosclerosis, inflammation, and lipid metabolism. J Clin Invest 108(6) 785-791 (2001).

5 Han, J., Hajjar, D.P., Febbraio, M., et al. Native and modified low density lipoproteins increase the functional expression of the macrophage class B scavenger receptor, CD36. J Biol Chem 272(34) 21654-21659 (1997).

6 Tontonoz, P., Nagy, L., Alvarez, J.G.A., et al. PPARγ promotes monocyte/macrophage differentiation and uptake of oxidized LDL. Cell 93 241-252 (1998).

7 Greenwalt, D.E., Lipsky, R.H., Ockenhouse, C.F., et al. Membrane glycoprotein CD36: A review of its roles in adherence, signal transduction, and transfusion medicine. Blood 80(5) 1105-1115 (1992).

8 Finnemann, S.C., and Silverstein, R.L. Differential roles of CD36 and αvβ5 integrin in photoreceptor phagocytosis by the retinal pigment epithelium. J Exp Med 194(9) 1289-1298 (2001).

9 Navazo, M.D.P., Daviet, L., Savill, J., et al. Identification of a domain (155-183) on CD36 implicated in the phagocytosis of apoptotic neutrophils. J Biol Chem 271(26) 15381-15385 (1996).

10 Jiménez, B., Volpert, O.V., Crawford, S.E., et al. Signals leading to apoptosis-dependent inhibition of neovascularization by thrombospondin-1. Nat Med 6(1) 41-48 (2000).

11 Rutella, S., Rumi, C., Puggioni, P., et al. Expression of thrombospondin receptor (CD36) in B-cell chronic lymphocytic leukemia as an indicator of tumor cell dissemination. Haematologica 84 419-424 (1999).