Antigen: human BLT₁ receptor amino acids 331-352 (ALEPGPSESLTASSPLKLNELN) · Host: rabbit · Application(s): CF, FC, IHC, and WB · The leukotriene B₄ receptor 1 (BLT₁ receptor), cloned from HL-60 human leukemia cells, has 352 amino acids and seven putative membrane-spanning domains.¹ The primary structure of the receptor is identical to that of a putative purinoceptor, P2Y7, which binds to micromolar concentrations of ATP.² Northern blotting reveals that the BLT₁ receptor is highly expressed in leukocytes, U937 cells, and to a much lower extent in spleen and thymus.¹ Sheep lung membranes have also been identified as a rich source for receptor isolation and purification.³ A second LTB₄ receptor, BLT₂, has recently been cloned and characterized.^4,5,6 Cayman Chemical’s BLT₁ receptor polyclonal antiserum is made against a peptide from the C-terminus of the BLT₁ receptor, which is located on the intracellular side of the plasma membrane. Therefore, when performing studies on whole cells, permeabilization of the cells is required for the antibody to enter the cytosol. The antiserum is recommended for immunohistochemical analysis of BLT₁ receptor expression in tissues of human and bovine origin. Successful recognition of the overexpression of BLT₁ in pancreatic ductal cells of human chronic pancreatitis and pancreatic cancer patients has been documented using this antiserum.¹

1 Yokomizo, T., Izumi, T., Chang, K., et al. A G-protein-coupled receptor for leukotriene B₄ that mediates chemotaxis. Nature 387 620-624 (1997).

2 Akbar, G.K.M., Dasari, V.R., Webb, T.E., et al. Molecular cloning of a novel P₂ purinoceptor from human erythroleukemia cells. J Biol Chem 271 18363-18367 (1996).

3 Votta, B., Keefer, J., and Mong, S. Characterization of the soluble leukotriene B₄ receptor from sheep lung membranes. Biochem J 270 213-218 (1990).

4 Yokomizo, T., Kato, K., Terawaki, K., et al. A second leukotriene B₄ receptor, BLT₂: A new therapeutic target in inflammation and immunological disorders. J Exp Med 193 421-431 (2000).

5 Kamohara, M., Takasaki, J., Matsumoto, M., et al. Molecular cloning and characterization of another leukotriene B₄ receptor. J Biol Chem 275 27000-27004 (2000).

6 Wang, S., Gustafson, E., Pang, L., et al. A novel hepatointestinal leukotriene B₄ receptor. J Biol Chem 275 40686-40694 (2000).