Hepsin Blocking Peptide (aa 241-260) || Cayman Chemical

Each vial contains 200 µg of lyophilized peptide consisting of human hepsin amino acids 241-260 (GGYLPFRDPNSEENSNDIAL). This peptide can be used in conjunction with Cayman’s hepsin polyclonal antibody - affinity-purified (aa 2416-260) (Catalog No. 100022) to block protein-antibody complex formation during analysis for hepsin. · Hepsin is a type II membrane-associated protein that has a extracellular proteolytic domain and exhibits low sequence homology to other known proteases.^1,2 Recently, hepsin overexpression was observed in prostate, breast, kidney, and ovarian cancers and due to low homology to other known proteases may provide a unique target for pharmacological therapy.^3,4,5,6,7 Hepsin is necessary for cell growth in vitro⁸ and may play a role in metastatic expansion by factor VII activation.^9,6 The observed molecular mass of hepsin by immunoblotting is 45.3 kDa. Cloning and characterization of murine and rat hepsin indicate 88% overall homology with human hepsin.^10,11,12 Northern blotting reveals high levels of hepsin transcripts in liver.^2,7

1 Tsuji, A., Torres-Rosado, A., Arai, T., et al. Hepsin, a cell membrane-associate protease. Characterization, tissue distribution, and gene localization. J Biol Chem 266(25) 16948-16953 (1991).

2 Leytus, S.P., Kurachi, K., Hagen, F.S., et al. A novel trypsin-like serine protease (hepsin) with a putative transmembrane domain expressed by human liver and hepatoma cells. Biochemistry 27 1067-1074 (1988).

3 Dhanasekaran, S.M., Barrette, T.R., Ghosh, D., et al. Delineation of prognostic biomarkers in prostate cancer. Nature 412 822-826 (2001).

4 Magee, J.A., Araki, T., Patil, S., et al. Expression profiling reveals hepsin overexpression in prostate cancer. Cancer Res 61 5692-5696 (2001).

5 Welsh, J.B., Sapinoso, L.M., Su, A.I., et al. Analysis of gene expression identifies candidate markers and pharmacological targets in prostate cancer. Cancer Res 61 5974-5978 (2001).

6 Zacharski, L.R. Expression of the factor VII activating protease, hepsin, in situ in renal cell carcinoma. Thromb Haemost 79 876-877 (1998).

7 Tanimoto, H., Yan, Y., Clarke, J., et al. Hepsin, a cell surface serine protease identified in hepatoma cells, is overexpressed in ovarian cancer. Cancer Res 57 2884-2887 (1997).

8 Torres-Rosado, A., O'Shea, K.S., Tsuji, A., et al. Hepsin, a putative cell-surface protease, is required for mammalian cell growth. Proc Natl Acad Sci USA 90 7181-7185 (1993).

9 Kazama, Y., Hamamoto, T., Foster, D.C., et al. Hepsin, a putative membrane-associated serine protease, activates human factor VII and initiates a pathway of blood coagulation on the cell surface leading to thrombin formation. J Biol Chem 270(1) 66-72 (1995).

10 Vu, T.H., Liu, R.W., Haaksman, C.J., et al. Identification and cloning of the membrane-associated serine protease, hepsin, from mouse preimplantation embryos. J Biol Chem 272(50) 31315-31320 (1997).

11 Kawamura, S., Kurachi, S., Deyashiki, Y., et al. Complete nucleotide sequence, origin of isoform and functional characterization of the mouse hepsin gene. Eur J Biochem 262 755-764 (1999).

12 Farley, D., Reymond, F., and Nick, H. Cloning and sequence analysis of rat hepsin, a cell surface serine proteinase. Biochim Biophys Acta 1173(3) 350-352 (1993).

Hepsin Blocking Peptide (aa 241-260)

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Hepsin Blocking Peptide (aa 241-260)

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