Antigen: human 5-OxoETE receptor C-terminal amino acids 408-423 · Host: rabbit · Application(s): ICC and WB · 5-Oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid (5-OxoETE) is a potent stimulator of chemotaxis for eosinophils and neutrophils that mediates its action via a putative G-protein coupled receptor (GPCR).^1,2 The 5-OxoETE receptor couples to G_i/o to inhibit cyclic AMP production and to mobilize intracellular calcium.^1,2 Northern blot analysis reveals that the receptor is highly expressed on eosinophils and neutrophils, suggesting a key role in the inflammatory response.² In addition, the protein can also be detected in tissues such as kidney and liver.^1,2 Cayman Chemical’s 5-OxoETE receptor polyclonal antibody can be used in western blot analysis (detection at 48 kDa) and immunocytochemistry. The high expression level of the receptor in leukocytes makes it the best positive control sample. When probing other tissue samples such as liver and kidney, membrane preparations or higher sensitivity detection systems may be required for visualization on immunoblot.

1 Hosoi, T., Koguchi, Y., Sugikawa, E., et al. Identification of a novel human eicosanoid receptor coupled to G_i/o. J Biol Chem 277 (35) 31459-31465 (2002).

2 Jones, C.E., Holden, S., Tenaillon, L., et al. Expression and characterization of a 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid receptor highly expressed on human eosinophils and neutrophils. Mol Pharmacol 63(3) 471-477 (2003).