Antigen: human ACAT-2 amino acids 3-20 (PGGARLRLQRTEGLGGER) · Host: rabbit · Application(s): WB, ICC, and IHC · Acyl-Coenzyme A: cholesterol acyltransferase-2 (ACAT-2) catalyzes the formation of cholesterol esters from cholesterol and long chain fatty acyl-coenzyme A. It is associated with lipoprotein particle secretion and thus plays an important role in the metabolism of triglyceride-rich lipoproteins like ApoB100.^1,2,3 In humans, ACAT-2 is primarily expressed in the small intestine, whereas in other mammals, the enzyme is expressed in the liver and the small intestine.^4,2 Human ACAT-2 has 522 amino acid with an estimated molecular weight of 60 kDa.⁵ Cayman’s antibody detects ACAT-2 at about 60 kDa in mouse liver, intestine, and fibroblasts.⁶

1 Rudel, L.L., Lee, R.G., and Cockman, T.L. Acyl coenzyme A: Cholesterol acyltransferase types 1 and 2: structure and function in atherosclerosis. Curr Opin Lipidol 12 121-127 (2001).

2 Katsuren, K., Tamura, T., Arashiro, R., et al. Structure of the human acyl-CoA: Cholesterol acyltransferase-2 (ACAT-2) gene and its relation to dyslipidemia. Biochim Biophys Acta 1531 230-240 (2001).

3 Lee, R.G., Willingham, M.C., Davis, M.A., et al. Differential expression of ACAT1 and ACAT2 among cells within liver, intestine, kidney, and adrenal of nonhuman primates. J Lipid Res 41 1991-2001 (2000).

4 Cases, S., Novak, S., Zheng, Y., et al. ACAT-2, a second mammalian acyl-CoA: Cholesterol acyltransferase. Its cloning, expression, and characterization. J Biol Chem 273(4) 26755-26764 (1998).

5 Oelkers, P., Behari, A., Cromley, D., et al. Characterization of two human genes encoding acyl coenzyme A: Cholesterol acyltransferase-related enzymes. J Biol Chem 273(41) 26765-26771 (1998).

6 Anderson, R.A., Joyce, C., Davis, M., et al. Identification of a form of acyl-CoA: Cholesterol acyltransferase specific to liver and intestine in nonhuman primates. J Biol Chem 273(41) 26747-26754 (1998).