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Antigen:
human ACAT-1 amino acids 6-23 (KMSLRNRLSKSRENPEED)
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Host:
rabbit
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Application(s):
WB, IHC, and ICC; other applications not tested
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Acyl-coenzyme A: cholesterol acyltransferase-1 (ACAT-1) catalyzes the formation of cholesterol esters from cholesterol and long chain fatty acyl-coenzyme A, and may play a role in the development of atherosclerosis.1,2 ACAT-1 is ubiquitously expressed, with highest levels observed in sebaceous glands, steroidogenic tissues, and macrophages.3,4,5 Human ACAT-1 has 550 amino acids and an estimated molecular weight of 65 kDa.5 Cayman’s affinity-purified antibody recognizes a 50 kDa band in most cultured cell lines as reported in the literature.6,7,4 In addition, the antibody reacts with a native form of ACAT-1 at about 65 kDa in liver tissues. Cayman Chemical’s affinity-purified ACAT-1 polyclonal antibody can be used for western blot, immunocytochemistry, and immunohistochemical analysis for ACAT-1 on samples of human, murine, rat, and porcine origin. Other applications for use of this antibody have not yet been tested.
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1
Rudel, L.L., Lee, R.G., Cockman, T.L. Acyl coenzyme A: Cholesterol acyltransferase types 1 and 2: structure and function in atherosclerosis. Curr Opin Lipidol 12 121-127 (2001).
2
Linton, M.F., Fazio, S. Macrophages, inflammation, and atherosclerosis. Int J Obes 27 S35-S40 (2003).
3
Namatame, I., Tomoda, H., Ishibashi, S., et al. Antiatherogenic activity of fungal beauveriolides, inhibitors of lipid droplet accumulation in macrophages. Proc Natl Acad Sci USA 101(3) 737-742 (2004).
4
Lee, R.G., Willingham, M.C., Davis, M.A., et al. Differential expression of ACAT1 and ACAT2 among cells within liver, intestine, kidney, and adrenal of nonhuman primates. J Lipid Res 41 1991-2001 (2000).
5
Chang, C.C.Y., Huh, H.Y., Cadigan, K.M., et al. Molecular cloning and functional expression of human acyl-coenzyme A: Cholesterol acyltransferase cDNA in mutant chinese hamster ovary cells. J Biol Chem 268(28) 20747-20755 (1993).
6
Chang, C.C.Y., Lee, C.G., Chang, E.T., et al. Recombinant acyl-CoA: cholesterol acyltransferase-1 (ACAT-1) purified to essential homogeneity utilizes cholesterol in mixed micelles or in vesicles in a highly cooperative manner. J Biol Chem 273(52) 35132-35141 (1998).
7
Chang, C.C.Y., Chen, J., Thomas, M.A., et al. Regulation and immunolocalization of acyl-coenzyme A: Cholesterol acytransferase in mammalian cells as studies with specific antibodies. J Biol Chem 270(49) 29532-29540 (1995).
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