Antigen: human endothelial lipase amino acids 19-32 · Host: rabbit · Application(s): WB and IHC · Endothelial lipase (EL) is a major genetic determinant for the concentration, structure, and metabolism of high-density lipoprotein, which protects against atherosclerosis.^1,2 It was originally cloned from endothelial cells and found to be expressed in a distinct and complementary tissue-restricted fashion, with high-level expression in the liver, placenta, lung, ovary, and macrophage.³ Immunohistochemical studies demonstrate that EL is expressed in infiltrating cells such as macrophages within atheromatous plaques, in addition to endothelial and smooth muscle cells in non-atherosclerotic coronary arteries. Furthermore, EL expression is detected in the neovasculature within atheromatous plaques in atherosclerotic coronary arteries, indicating that EL may have unique functional roles in atherosclerosis.⁴ Cayman Chemical’s EL polyclonal antibody can be used for western blot and immunohistochemical analysis for EL on samples of human, murine, rat, porcine, and ovine origin. Other applications for use of this antibody have not yet been tested.

1 Ishida, T., Choi, S., Kundu, R.K., et al. Endothelial lipase is a major determinant of HDL level. J Clin Invest 111(3) 347-355 (2003).

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3 Hirata, K., Dichek, H.L., Cioffi, J.A., et al. Cloning of a unique lipase from endothelial cells extends the lipase gene family. J Biol Chem 274(20) 14170-14175 (2003).

4 Azumi, H., Hirata, K., Ishida, T., et al. Immunohistochemical localization of endothelial cell-derived lipase in atherosclerotic human coronary arteries. Cardiovasc Res 58 647-654 (2003).