Limit of detection: 80% B/B₀: 45 pg/ml · Sensitivity: 50% B/B₀: 176 pg/ml · Thromboxane A₂ (TXA₂) is produced from arachidonic acid by many cells and causes irreversible platelet aggregation and contraction of vascular and bronchial smooth muscle. ^1,2,3 TXA₂ is rapidly hydrolyzed non-enzymatically to TXB₂, which is then quickly metabolized to urinary metabolites for clearance by the kidneys. Urinary analysis of TXB₂ accurately reflects intrarenal TXA₂ synthesis,⁴ while measurement of 11-dehydro and 2,3-dinor thromboxane metabolites gives the best estimate of systemic TXA₂ secretion.^5,6 Cayman’s Thromboxane B₂ (TXB₂) Express EIA is a competitive assay that provides accurate measurements of TXB₂ from a variety of sample types. As the name implies, this kit was designed for rapid measurements of TXB₂. Features of the kit include: Fast - two hour incubation; 1 hour development · Standard curve range: 15.6 - 2,000 pg/ml · Patented AChE Tracer technology · Applicable to TXB₂ measurements in plasma, urine, and cell culture

1 Hamberg, M., Svensson, J., and Samuelsson, B. Thromboxanes: A new group of biologically active compounds derived from prostaglandin endoperoxides. Proc Natl Acad Sci USA 72 2994-2998 (1975).

2 Ellis, E.F., Oelz, O., Roberts, L.J., et al. Coronary arterial smooth muscle contraction by a substance released from platelets: Evidence that it is thromboxane A₂. Science 193 1135-1137 (1976).

3 Salzman, P.M., Salmon, J.A., and Moncada, S. Prostacyclin and thromboxane A₂ synthesis by rabbit pulmonary artery. J Pharmacol Exp Ther 215 240-247 (1980).

4 Patrono, C., Ciabattoni, G., Patrignani, P., et al. Evidence for a renal origin of urinary thromboxane B₂ in health and disease. Adv Prostaglandin Thromboxane Leukot Res 11 493-498 (1983).

5 Lawson, J.A., Patrono, C., Ciabattoni, G., et al. Long-lived enzymatic metabolites of thromboxane B₂ in the human circulation. Anal Biochem 155 198-205 (1986).

6 Patrono, C., Ciabattoni, G., Pugliese, F., et al. Estimated rate of thromboxane secretion into the circulation of normal humans. J Clin Invest 77 590-594 (1986).

Hamberg, M., Svensson, J., and Samuelsson, B. Thromboxanes: A new group of biologically active compounds derived from prostaglandin endoperoxides. Proc Natl Acad Sci USA 72 2994-2998 (1975).

Patrono, C., Ciabattoni, G., Pugliese, F., et al. Estimated rate of thromboxane secretion into the circulation of normal humans. J Clin Invest 77 590-594 (1986).

Patrono, C., Ciabattoni, G., Patrignani, P., et al. Evidence for a renal origin of urinary thromboxane B₂ in health and disease. Adv Prostaglandin Thromboxane Leukot Res 11 493-498 (1983).

Ellis, E.F., Oelz, O., Roberts, L.J., et al. Coronary arterial smooth muscle contraction by a substance released from platelets: Evidence that it is thromboxane A₂. Science 193 1135-1137 (1976).

Salzman, P.M., Salmon, J.A., and Moncada, S. Prostacyclin and thromboxane A₂ synthesis by rabbit pulmonary artery. J Pharmacol Exp Ther 215 240-247 (1980).

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