Antigen: human 11β-HSD1 amino acids 77-92 (CLELGAASAHYIAGT) · Host: rabbit · Application(s): WB and IHC (formalin-fixed paraffin-embedded sections); other applications not tested · 11β-Hydroxysteroid dehydrogenase (Type 1) (11β-HSD1) catalyzes the conversion of inactive cortisone to active cortisol in adipose tissue. mRNA and protein for this enzyme have been detected in a wide range of tissues including liver, kidney, testis, lung, heart, and colon.¹ The enzyme’s expression is developmentally regulated, suggesting its importance in regulation of glucocorticoid action during embryonic development.² Over-expression of 11β-HSD1 results in visceral obesity and metabolic syndrome including insulin-resistant diabetes, hyperlipidemia, and hyperphagia.³ Cayman Chemical’s 11β-HSD1 Polyclonal Antibody can be used for WB and IHC (formalin-fixed paraffin-embedded sections) analysis for 11β-HSD1 on samples of human, murine, and rat origin. Other applications for use of this antibody have not yet been tested. The antibody detects a 32 kDa protein in human, mouse, and rat liver samples by WB analysis.

1 White, P.C., Mune, T., and Agarwal, A.K. 11β-hydroxysteroid dehydrogenase and the syndrome of apparent mineralocorticoid excess. Endocr Rev 18(1) 135-156 (1997).

2 Thompson, A., Han, V.K.M., and Yang, K. Differential expression of 11β-hydroxysteroid dehydrogenase types 1 and 2 mRNA and glucocorticoid receptor protein during mouse embryonic development. J Steroid Biochem Mol Biol 88 367-375 (2004).

3 Masuzaki, H., Paterson, J., Shinyama, H., et al. A transgenic model of visceral obesity and the metabolic syndrome. Science 294 2166-2170 (2001).

Ricketts, M.L., Verhaeg, J.M., Bujalska, I., et al. Immunohistochemical localization of type 1 11β-hydroxysteroid dehydrogenase in human tissue. J Clin Endocrinol Metab 83(4) 1325-1335 (1998).

Masuzaki, H., Paterson, J., Shinyama, H., et al. A transgenic model of visceral obesity and the metabolic syndrome. Science 294 2166-2170 (2001).

Thompson, A., Han, V.K.M., and Yang, K. Differential expression of 11β-hydroxysteroid dehydrogenase types 1 and 2 mRNA and glucocorticoid receptor protein during mouse embryonic development. J Steroid Biochem Mol Biol 88 367-375 (2004).

White, P.C., Mune, T., and Agarwal, A.K. 11β-hydroxysteroid dehydrogenase and the syndrome of apparent mineralocorticoid excess. Endocr Rev 18(1) 135-156 (1997).

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