Antigen: recombinant human L-PGD synthase · Clone designation: 10A5 · Host: rat · Isotype: IgG1κ · Application(s): WB and IHC · Prostaglandin D synthase (PGDS) catalyzes the isomerization of PGH₂ to produce PGD₂. PGD₂ induces sleep, regulates nociception, inhibits platelet aggregation, and acts as an allergic mediator. Two distinct types of PGDS have been identified, namely the lipocalin type enzyme (β-trace) and the hematopoietic enzyme.^1,2 Lipocalin-type PGDS is localized in the central nervous system, genital organs of various mammals and the human heart.^1,3,4,5 Patients with chronic renal failure and hypertension exhibit elevated amounts of lipocalin-type PGDS in serum and urine.⁶ The lipocalin-type PGDS has been identified as β-trace (~24 kDa), which is a major protein in the human cerebrospinal fluid.^2,7

1 Urade, Y., Watanabe, K., and Hayaishi, O. Prostaglandin D, E, and F synthases. J Lipid Mediat Cell Signal 12 257-273 (1995).

2 Toh, H., Kubodera, H., Nakajima, N., et al. Glutathione-independent prostaglandin D synthase as a lead molecule for designing new functional proteins. Protein Eng 9 1067-1082 (1996).

3 Tokugawa, Y., Kunishige, I., Kuboto, Y., et al. Lipocalin-type prostaglandin D synthase in human male reproductive organs and seminal plasma. Biol Reprod 58 600-607 (1998).

4 Melegos, D.N., Diamandis, E.P., Oda, H., et al. Immunofluorometric assay of prostaglandin D synthase in human tissue extracts and fluids. Clin Chem 42 1984-1991 (1996).

5 Kanaoka, Y., Fujimori, K., Kikuno, R., et al. Structure and chromosomal localization of human and mouse genes for hematopoietic prostaglandin D synthase. Eur J Biochem 267 3315-3322 (2000).

6 Hirawa, N., Uehara, Y., Yamakado, M., et al. Lipocalin-type prostaglandin D synthase in essential hypertension. Hypertension 39 (part 2) 449-454 (2002 Dec 12).

7 Zahn, M., Mäder, A., Schmidt, B., et al. Purification and N-terminal sequence of β-trace, a protein abundant in human cerebrospinal fluid. Neurosci Lett 154 93-95 (1993).