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Prostaglandin D Synthase (hematopoietic-type; mouse recombinant) Exclusive

Cayman Chemical Item Number 10004347

H-PGDS; Hematopoietic-PGDS

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Description

Source: active mouse recombinant enzyme purified from E. coli overexpression system · Mr: 23.5 kDa · Prostaglandin D synthase (PGDS) catalyzes the isomerization of PGH2 to produce PGD2. PGD2 induces sleep, regulates nociception, inhibits platelet aggregation, and acts as an allergic mediator. Two distinct types of PGDS have been identified, namely the lipocalin-type enzyme (b-trace) and the hematopoietic enzyme.1,2,3,4 Lipocalin-type PGDS is localized in the central nervous system and male genital organs of various mammals and the human heart. This enzyme has been identified as b-trace, which is a major protein in human cerebrospinal fluid.3,4 Hematopoietic-PGDS is widely distributed in the peripheral tissues and is localized in the antigen-presenting cells, mast cells, and megakaryocytes.1 This enzyme, which requires glutathione for activity, belongs to the sigma-class of glutathione-S-transferases and is 23.5 kDa in size.1,5,4

1 Kanaoka, Y., Fujimori, K., Kikuno, R., et al. Structure and chromosomal localization of human and mouse genes for hematopoietic prostaglandin D synthase. Eur J Biochem 267 3315-3322 (2000).

2 Jowsey, I.R., Thomson, A.M., Flanagan, J.U., et al. Mammalian class sigma glutathione s-transferases: Catalytic properties and tissue-specific expression of human and rat GSH-dependent prostaglandin D2 synthases. Biochem J 359 507-516 (2001).

3 Urade, Y., Watanabe, K., and Hayaishi, O. Prostaglandin D, E, and F synthases. J Lipid Mediat Cell Signal 12 257-273 (1995).

4 Kanaoka, Y., Ago, H., Inagaki, E., et al. Cloning and crystal structure of hematopoietic prostaglandin D synthase. Cell 90 1085-1095 (1997).

5 Urade, Y., Fujimoto, N., Ujihara, M., et al. Biochemical and immunological characterization of rat spleen prostaglandin D synthetase. J Biol Chem 262(8) 3820-3825 (1987).

Synonyms
  • H-PGDS
  • Hematopoietic-PGDS
Formulation 50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride, 0.5 mM EDTA, and 50% glycerol
Purity ≥85%
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Kanaoka, Y., Fujimori, K., Kikuno, R., et al. Structure and chromosomal localization of human and mouse genes for hematopoietic prostaglandin D synthase. Eur J Biochem 267 3315-3322 (2000).

Urade, Y., Fujimoto, N., Ujihara, M., et al. Biochemical and immunological characterization of rat spleen prostaglandin D synthetase. J Biol Chem 262(8) 3820-3825 (1987).

Jowsey, I.R., Thomson, A.M., Flanagan, J.U., et al. Mammalian class sigma glutathione s-transferases: Catalytic properties and tissue-specific expression of human and rat GSH-dependent prostaglandin D2 synthases. Biochem J 359 507-516 (2001).

Urade, Y., Watanabe, K., and Hayaishi, O. Prostaglandin D, E, and F synthases. J Lipid Mediat Cell Signal 12 257-273 (1995).

Kanaoka, Y., Ago, H., Inagaki, E., et al. Cloning and crystal structure of hematopoietic prostaglandin D synthase. Cell 90 1085-1095 (1997).

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Size Price Quantity Subtotal
50 µg $105.00 $0.00
100 µg $200.00 $0.00
250 µg $473.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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