Antigen: recombinant human mPGE synthase-1 · Clone designation: 6C6 · Host: mouse · Application(s): WB, ICC, and IHC · Prostaglandin E synthase-1 (PGE synthase-1) catalyzes the isomerization of PGH₂ to PGE₂. PGE₂ is one of the primary cyclooxygenase products of arachidonic acid and one of the most widely investigated prostaglandins. Its activity influences inflammation, fertility and parturition, gastric mucosal integrity, and immune modulation.^1,2,3,4 PGE synthase-1 is a 16 kDa membrane associated protein with glutathione-dependent activity.^5,6 The mRNA for PGE synthase-1 is expressed in a variety of tissues including prostate, testes, and small intestine as well as in A549 and HeLa cells. PGE synthase-1 protein expression is increased in A549 cells following treatment with IL-1β.⁵ Cayman Chemical’s PGE synthase-1 (microsomal) monoclonal antibody can be used for western blot, immunocytochemistry, and immunohistochemical (formalin-fixed paraffin-embedded tissue) analysis for mPGE synthase-1 on samples of human origin. Other applications for use of this antibody have not yet been tested.

1 Willis, A.L., and Cornelsen, M. Repeated injection of prostaglandin E₂ in rat paws induces chronic swelling and a marked decrease in pain threshold. Prostaglandins 3 353-357 (1973).

2 Jackson, G.M., Sharp, H.T., and Varner, M.W. Cervical ripening before induction of labor: A randomized trial of prostaglandin E₂ gel versus low-dose oxytocin. Am J Obstet Gynecol 171 1092-1096 (1994).

3 Robert, A., Schultz, J.R., Nezamis, J.E., et al. Gastric antisecretory and antiulcer properties of PGE₂, 15-methyl PGE₂, and 16,16-dimethyl PGE₂. Intravenous, oral and intrajejunal administration. Gastroenterology 70 359-370 (1976).

4 Arvind, P., Papavassiliou, E.D., Tsioulias, G.J., et al. Prostaglandin E₂ down-regulates the expression of HLA-DR antigen in human colon adenocarcinoma cell lines. Biochemistry 34 5604-5609 (1995).

5 Jakobsson, P., Thorén, S., Morgenstern, R., et al. Identification of human prostaglandin E synthase: A microsomal, glutathione-dependent, inducible enzyme, constituting a potential novel drug target. Proc Natl Acad Sci USA 96 7220-7225 (1999).

6 Urade, Y., Watanabe, K., and Hayaishi, O. Prostaglandin D, E, and F synthases. J Lipid Mediat Cell Signal 12 257-273 (1995).

Willis, A.L., and Cornelsen, M. Repeated injection of prostaglandin E₂ in rat paws induces chronic swelling and a marked decrease in pain threshold. Prostaglandins 3 353-357 (1973).

Jackson, G.M., Sharp, H.T., and Varner, M.W. Cervical ripening before induction of labor: A randomized trial of prostaglandin E₂ gel versus low-dose oxytocin. Am J Obstet Gynecol 171 1092-1096 (1994).

Arvind, P., Papavassiliou, E.D., Tsioulias, G.J., et al. Prostaglandin E₂ down-regulates the expression of HLA-DR antigen in human colon adenocarcinoma cell lines. Biochemistry 34 5604-5609 (1995).

Jakobsson, P., Thorén, S., Morgenstern, R., et al. Identification of human prostaglandin E synthase: A microsomal, glutathione-dependent, inducible enzyme, constituting a potential novel drug target. Proc Natl Acad Sci USA 96 7220-7225 (1999).

Urade, Y., Watanabe, K., and Hayaishi, O. Prostaglandin D, E, and F synthases. J Lipid Mediat Cell Signal 12 257-273 (1995).

Robert, A., Schultz, J.R., Nezamis, J.E., et al. Gastric antisecretory and antiulcer properties of PGE₂, 15-methyl PGE₂, and 16,16-dimethyl PGE₂. Intravenous, oral and intrajejunal administration. Gastroenterology 70 359-370 (1976).

Show all 6 Hide all but first 3