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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Prostaglandin E2 FPIA Kit - Red

Cayman Chemical Item Number 10004517

PGE2 Fluorescence Polarization Immunoassay - Red

Prostaglandin E2 FPIA Kit - Red

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Description

Limit of detection: 100 pg/ml · Cayman Chemical’s Prostaglandin E2 (PGE2) Fluorescence Polarization Immunoassay (FPIA) is an excellent method for rapid, high-throughput screening of PGE2 samples. This assay utilizes the red-shifted dye rhodamine as the label, rather than fluorescein, which is used in our original PGE2 FPIA Kit - Green (Catalog No. 500501). Compared to “green” dyes absorbing at lower wavelength, such as fluorescein, red-shifted dyes offer the advantage of limited interference from most compounds in a drug screening library, as well as reduced interference from sample matrices. Samples from cell culture and recombinant protein expression systems may require modest dilutions (1:5-1:20) and no purification prior to being used. Samples containing no phenol red or serum can be assayed without any dilution. Using this assay it is possible to rapidly analyze samples and increase the rate of lead compound identification without time-consuming incubation, washing, and development steps and without complex interference problems. Each kit contains FPIA reagent, buffer concentrate (10X), standard, 384 well plate, foil plate cover, and complete instructions.
Synonyms
  • PGE2 Fluorescence Polarization Immunoassay - Red
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Prostaglandin E1 100%
Prostaglandin E2 100%
Prostaglandin E2 Ethanolamide 100%
Prostaglandin E3 85.0%
Sulprostone 9.0%
6-keto Prostaglandin F1α 2.9%
8-iso Prostaglandin F2α 0.09%
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Background Reading

Granström, E., Hamberg, M., Hansson, G., et al. Chemical instability of 15-keto-13,14-dihydro-PGE2: The reason for low assay reliability. Prostaglandins 19 933-945 (1980).

Hamberg, M., and Samuelsson, B. On the metabolism of prostaglandin E1 and E2 in the guinea pig. J Biol Chem 247 3495-3502 (1972).

Zhang, J., Chung, T.D.Y., and Oldenburg, K.R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 4(2) 67-73 (1999).

Fitzpatrick, F.A., Aguirre, R., Pike, J.E., et al. The stability of 13,14-dihydro-15 keto-PGE2. Prostaglandins 19 917-931 (1980).

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10004517-1920WELL
Prostaglandin E2 FPIA Reagent - Red 2000 dtn
Prostaglandin E2 FPIA Standard - Red 1 ea
384-Well Solid Plate (black; non-binding)
Foil Plate Covers
FPIA Buffer Concentrate (10X) 4 × 1 ea
10004517-384WELL
Prostaglandin E2 FPIA Reagent - Red 400 dtn
Prostaglandin E2 FPIA Standard - Red 1 ea
384-Well Solid Plate (black; non-binding)
Foil Plate Covers
FPIA Buffer Concentrate (10X) 2 × 1 ea
Size Price Quantity Subtotal
384 wells $305.00 $0.00
1920 wells $1,144.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Prostaglandin E2 FPIA Kit - Red under the Cayman Challenge program.

Placeholder for FPIA Red validation link text

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Is this kit right for my sample type? Prostaglandin E2 Recommendation Chart

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UltraPure Water
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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640

Copyright 2012 Cayman Chemical Company

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