Clone designation: C-11 · Host: Mouse · Application(s): WB, flow cytometry, ICC, and IHC (paraffin sections) · This pan-cytokeratin antibody detects cytokeratin isoforms 4, 5, 6, 8, 10, 13, and 18.¹ Cytokeratins make up a large family of proteins classifed as intermediate filament polypeptides.^1,2 They are subdivided into type I (acidic) and type II (basic/neutral) proteins.³ Under normal conditions, these proteins are found in epithelial cells as type I/type II pairs.^1,4 These pairs provide cellular support as well as contributing to more dynamic processes. They undergo extensive post translational modifications, such as phosphorylation, and are important for cancer diagnostics.^4,5 This antibody recognizes human cytokeratins 4, 5, 6, 8, 10, 13, and 18.¹ Accordingly the molecular migration is isoform dependent and expected to range from 40 to 70 kDa depending upon the cytokeratin composition of the sample.

1 Bártek, J., Vojtêšek, B., Stašková, Z., et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol 164 215-224 (1991).

2 Magin, T.M., Vijayaraj, P., and Leube, R.E. Structural and regulatory functions of keratins. Exp Cell Res 313 2021-2032 (2007).

3 Gu, L., and Coulombe, P.A. Keratin function in skin epithelia: A broadening palette with surprising shades. Curr Opin Cell Biol 19 13-23 (2007).

4 Ditzel, H.J., Strik, M.C.M., Larsen, M.K., et al. Cancer-associated cleavage of cytokeratin 8/18 heterotypic complexes exposes a neoepitope in human adenocarcinomas. J Biol Chem 277(24) 21712-21722 (2002).

5 Plowman, J.E. The proteomics of keratin proteins. J Chromatogr B 849 181-189 (2007).

Gu, L., and Coulombe, P.A. Keratin function in skin epithelia: A broadening palette with surprising shades. Curr Opin Cell Biol 19 13-23 (2007).

Ditzel, H.J., Strik, M.C.M., Larsen, M.K., et al. Cancer-associated cleavage of cytokeratin 8/18 heterotypic complexes exposes a neoepitope in human adenocarcinomas. J Biol Chem 277(24) 21712-21722 (2002).

Plowman, J.E. The proteomics of keratin proteins. J Chromatogr B 849 181-189 (2007).

Bártek, J., Vojtêšek, B., Stašková, Z., et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol 164 215-224 (1991).

Magin, T.M., Vijayaraj, P., and Leube, R.E. Structural and regulatory functions of keratins. Exp Cell Res 313 2021-2032 (2007).

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