CD95 Monoclonal Antibody (Clone IPO-4)

Cayman Chemical Item Number 10004604

APO-1; Fas; Tumor Necrosis Factor Receptor Superfamily, Member 6; TNFRSF6; Apoptosis Antigen; APT

Description

Clone designation: IPO-4 · Host: mouse · Application(s): ICC and IHC (frozen tissue sections), and flow cytometry; this antibody does not work for immunoblotting or IHC (paraffin-embedded sections) · CD95 is a type I transmembrane glycoprotein. It is expressed by activated T and B cells, Reed-Sternberg cells, Hodgkin’s cells, thymocytes, as well as heart, lung, liver, and ovarian cells. Fas-ligand (CD95L) or monoclonal antibodies bound to CD95 can induce apoptosis. Cayman Chemical’s CD95 monoclonal antibody can be used for immunocytochemical and immunohistochemical (IHC) of frozen tissue sections analysis for CD95 on samples of human origin. The antibody can also be used for flow cytometry and to induce apoptosis of cultured cell lines.¹ It does not work for immunoblotting or IHC on paraffin-embedded tissue sections. Other applications for use of this antibody have not yet been tested.

1 Li, J.H., Kluger, M.S., Madge, L.A., et al. Interferon-γ augments CD95(APO-1/Fas) and pro-caspase-8 expression and sensitizes human vascular endothelial cells to CD95-mediated apoptosis. Am J Pathol 161(4) 1485-1495 (2002).

Synonyms

APO-1
Fas
Tumor Necrosis Factor Receptor Superfamily, Member 6
TNFRSF6
Apoptosis Antigen
APT

Formulation

100 µg protein-a purified IgM in 100 µl PBS, pH 7.2

Stability

1 year

Storage

4°C

Shipping

Wet ice in continental US; may vary elsewhere

Specificity

Human CD95

Background Reading

Li, J.H., Kluger, M.S., Madge, L.A., et al. Interferon-γ augments CD95(APO-1/Fas) and pro-caspase-8 expression and sensitizes human vascular endothelial cells to CD95-mediated apoptosis. Am J Pathol 161(4) 1485-1495 (2002).