Clone designation: CB19 · Host: mouse · Application(s): ICC, IHC (frozen tissue sections), and flow cytometry · CD19 is a type I transmembrane glycoprotein important in B-lymphocyte signal transduction.¹ Together with CD21, CD81, and β1 integrin, it is a part of the B-lymphocyte signaling complex, which regulates B-cell activation and differentiation.² Originally identified as B₄, CD19 is a pan B-cell antigen expressed from the B-cell progenitor stage where rearrangement of immunoglobulin genes takes place, to the terminal stage of B-cell differentiation into plasma cells.^3,4,2 CD19 is detected on follicular dendritic cells and malignant B-cells. Cayman Chemical’s CD19 Monoclonal Antibody can be used for flow cytometry, immunocytochemistry, and immunohistochemistry (frozen tissue sections) on samples of human origin. This monoclonal antibody can be used to identify human leukemias and lymphomas. Other applications for use of this antibody have not yet been tested.

1 Tedder, T.F., and Isaacs, C.M. Isolation of cDNAs encoding the CD19 antigen of human and mouse B lymphocytes. A new member of the immunoglobulin superfamily. J Immunol 143(2) 712-717 (1989).

2 Sato, S., Steeber, D.A., and Tedder, T.F. The CD19 signal transduction molecule is a response regulator of B-lymphocyte differentiation. Proc Natl Acad Sci USA 92 11558-11562 (1995).

3 Nadler, L.M., Anderson, K.C., Marti, G., et al. B₄, a human B lymphocyte-associated antigen expressed on normal, mitogen-activated, and malignant B lymphocytes. J Immunol 131(1) 244-250 (1983).

4 Pezzutto, A., Dörken, B., Rabinovitch, P.S., et al. CD19 monoclonal antibody HD37 inhibits anti-immunoglobulin-induced B cell activation and proliferation. J Immunol 138(9) 2793-2799 (1987).

Tedder, T.F., and Isaacs, C.M. Isolation of cDNAs encoding the CD19 antigen of human and mouse B lymphocytes. A new member of the immunoglobulin superfamily. J Immunol 143(2) 712-717 (1989).

Nadler, L.M., Anderson, K.C., Marti, G., et al. B₄, a human B lymphocyte-associated antigen expressed on normal, mitogen-activated, and malignant B lymphocytes. J Immunol 131(1) 244-250 (1983).

Pezzutto, A., Dörken, B., Rabinovitch, P.S., et al. CD19 monoclonal antibody HD37 inhibits anti-immunoglobulin-induced B cell activation and proliferation. J Immunol 138(9) 2793-2799 (1987).

Sato, S., Steeber, D.A., and Tedder, T.F. The CD19 signal transduction molecule is a response regulator of B-lymphocyte differentiation. Proc Natl Acad Sci USA 92 11558-11562 (1995).

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