PCNA Monoclonal Antibody (Clone IPO-38)

Cayman Chemical Item Number 10004805

Nuclear Antigen of Proliferating Cells; Proliferating Cell Nuclear Antigen

Description

Clone designation: IPO-38 · Host: mouse · Application(s): WB, flow cytometry, ICC, and IHC (frozen and paraffin-embedded sections) · The proliferating cell nuclear antigen (PCNA) becomes detectable in the G₁ phase of the cell cycle and reaches a maximum level of expression during mitosis. PCNA is highly conserved across species and is a critical part of the DNA polymerase δ holoenzyme.^1,2,3 The level of PCNA expression depends on the proliferative potential of the cell examined. Cayman Chemical’s PCNA monoclonal antibody can be used for analysis of PCNA by western blot, flow cytometry, immunocytochemistry, and immunohistochemistry (frozen and paraffin-embedded tissue) on samples from multiple species. This monoclonal antibody stains proliferating cells in a wide range of normal tissues, as well as in tissues undergoing pathological processes and after mitogenic and allogeneic stimulations.

1 Tan, C., Castillo, C., So, A.G., et al. An auxiliary protein for DNA polymerase-δ from fetal calf thymus. J Biol Chem 261(26) 12310-12316 (1986).

2 Fukuda, K., Morioka, H., Imajou, S., et al. Structure-function relationship of the eukaryotic DNA replication factor, proliferating cell nuclear antigen. J Biol Chem 270(38) 22527-22534 (1995).

3 Travali, S., Ku, D., Rizzo, M.G., et al. Structure of the human gene for the proliferating cell nuclear antigen. J Biol Chem 264(13) 7466-7472 (1989).

Synonyms

Nuclear Antigen of Proliferating Cells
Proliferating Cell Nuclear Antigen

Formulation

100 µg purified IgM in 200 µl PBS, pH 7.2, containing 0.02% sodium azide

Stability

1 year

Storage

4°C

Shipping

Wet ice in continental US; may vary elsewhere

Specificity

Multiple species PCNA (species- and tissue-unspecific)

Background Reading

Tan, C., Castillo, C., So, A.G., et al. An auxiliary protein for DNA polymerase-δ from fetal calf thymus. J Biol Chem 261(26) 12310-12316 (1986).

Travali, S., Ku, D., Rizzo, M.G., et al. Structure of the human gene for the proliferating cell nuclear antigen. J Biol Chem 264(13) 7466-7472 (1989).

Fukuda, K., Morioka, H., Imajou, S., et al. Structure-function relationship of the eukaryotic DNA replication factor, proliferating cell nuclear antigen. J Biol Chem 270(38) 22527-22534 (1995).

Size

Price

Quantity

Subtotal

1 ea

$182.00

$0.00

Bulk

Contact

Cart Total

$0.00