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sPLA2 (Type V) Inhibitor Screening Assay Kit

Cayman Chemical Item Number 10004883

Secretory Phospholipase A2 (Type V)

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Description

Phospholipase A2 (PLA2) catalyzes the hydrolysis of phospholipids at the sn-2 position yielding a free fatty acid and a lysophospholipid. The release of arachidonic acid from membrane phospholipids by PLA2 is believed to be a key step in the control of eicosanoid production within the cell.1 Mammalian tissues contain many secretory PLA2s (sPLA2s), including types IB, IIA-F, III, V, X, and XII2 of which Type V sPLA2 is involved in eicosanoid formation in inflammatory cells, such as macrophages and mast cells.3,4 It has been demonstrated that human type V sPLA2 can bind phosphatidylcholine (PC) membranes and hydrolyze PC substrates much more efficiently than human type IIA sPLA2, which makes it better suited for acting on the outer plasma membrane.5 Selective targeting and inhibition of sPLA2s has been problematic as evidenced by the lack of availability of isozyme-specific inhibitors. The Cayman Chemical sPLA2 (Type V) Inhibitor Screening Assay is a convenient colorimetric assay designed for rapid screening of Type V sPLA2 inhibitors in a 96 well format. The sPLA2 (Type V) Inhibitor Screening assay kit includes sufficient human recombinant Type V sPLA2 for a minimum of 100 reactions. Identification of selective sPLA2 inhibitors will offer substantial aid in the elucidation of the specific physiological function of each sPLA2. Each kit contains assay buffer, DTNB, diheptanoyl thio-PC, human sPLA2, a 96 well plate, plate cover, and complete instructions.

1 Dennis, E.A. Diversity of group types, regulation, and function of phospholipase A2. J Biol Chem 269 13057-13060 (1994).

2 Balboa, M.A., Balsinde, J., Winstead, M.V., et al. Novel group V phospholipase A2 involved in arachidonic acid mobilization in murine P388D1 macrophages. J Biol Chem 271 32381-32384 (1996).

3 Reddy, S.T., Winstead, M.V., Tischfield, J.A., et al. Analysis of the secretory phospholipase A2 that mediates prostaglandin production in mast cells. J Biol Chem 272 13591-13596 (1997).

4 Han, S., Yoon, E.T., and Cho, W. Bacterial expression and characterization of human secretory class V phospholipase A2. Biochem J 331 353-357 (1998).

5 Han, S.K., Kim, K.P., Koduri, R., et al. Roles of Trp31 in high membrane binding and proinflammatory activity of human group V phospholipase A2. J Biol Chem 274 11881-11888 (1999).

Synonyms
  • Secretory Phospholipase A2 (Type V)
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Dennis, E.A. Phospholipases. Enzymes XVI 307-353 (1983).

Reynolds, L.J., Hughes, L.L., and Dennis, E.A. Analysis of human synovial fluid phospholipase A2 on short chain phosphatidylcholine-mixed micelles: Development of a spectrophotometric assay suitable for a microtiterplate reader. Anal Biochem 204 190-197 (1992).

Hendrickson, H.S., Hendrickson, E.K., and Dybvig, R.H. Chiral synthesis of a dithiolester analog of phosphatidylcholine as a substrate for the assay of phospholipase A2. J Lipid Res 24 1532-1537 (1983).

Balboa, M.A., Balsinde, J., Winstead, M.V., et al. Novel group V phospholipase A2 involved in arachidonic acid mobilization in murine P388D1 macrophages. J Biol Chem 271 32381-32384 (1996).

Reddy, S.T., Winstead, M.V., Tischfield, J.A., et al. Analysis of the secretory phospholipase A2 that mediates prostaglandin production in mast cells. J Biol Chem 272 13591-13596 (1997).

Han, S.K., Kim, K.P., Koduri, R., et al. Roles of Trp31 in high membrane binding and proinflammatory activity of human group V phospholipase A2. J Biol Chem 274 11881-11888 (1999).

Han, S., Yoon, E.T., and Cho, W. Bacterial expression and characterization of human secretory class V phospholipase A2. Biochem J 331 353-357 (1998).

Dennis, E.A. Diversity of group types, regulation, and function of phospholipase A2. J Biol Chem 269 13057-13060 (1994).

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10004883-96WELL
sPLA2 Assay (human Type V) 1 ea
96-Well Solid Plate (Colorimetric Assay)
sPLA2 Assay Buffer (10X) 1 ea
sPLA2 DTNB 4 × 1 ea
sPLA2 Diheptanoyl thio-PC (substrate) 2 × 1 ea
Size Price Quantity Subtotal
96 wells $348.00 $0.00
Bulk Contact
Cart Total $0.00

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Pricing updated 2012-05-25. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample sPLA2 (Type V) Inhibitor Screening Assay Kit under the Cayman Challenge program.

Related Products

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sPLA2 (human Type IIA) EIA Kit
sPLA2 (human type V) Western Ready Control
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