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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Mouse Macrophage Microsomal Immunoblot Control

Cayman Chemical Item Number 10004910

Mouse macrophage microsomes protein

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Description

Cyclooxygenase-2 (COX-2) is a 72 kDa protein that has been cloned from a variety of species including human, mouse, rat, and ovine.1,2,3,4,5 This preparation of COX-2 microsomes was prepared by solubilization and dilution of the 100,000 x g pellet from the lysate of cytokine-stimulated mouse macrophage cultures. Cayman Chemical’s preparation of mouse macrophage microsomes is a ready-to-use sample for SDS-PAGE. As such, it is an excellent positive control for the following Cayman Chemical polyclonal antibodies: Item Nos. 160106, 160116, 160126, and 101750.

1 Smith, W.L., Garavito, R.M., and DeWitt, D.L. Prostaglandin endoperoxide H synthases (cyclooxygenases)-1 and -2. J Biol Chem 271 33157-33160 (1996).

2 Hla, T., and Neilson, K. Human cyclooxygenase-2 cDNA. Proc Natl Acad Sci USA 89 7384-7388 (1992).

3 Kujubu, D.A., Fletcher, B.S., Varnum, B.C., et al. TIS10, a phorbol ester tumor promoter-inducible mRNA from Swiss 3T3 cells, encodes a novel prostaglandin synthase/cyclooxygenase homologue. J Biol Chem 266 12866-12872 (1991).

4 Yamagata, K., Andreasson, K.I., Kaufmann, W.E., et al. Expression of a mitogen-inducible cyclooxygenase in brain neurons: Regulation by synaptic activity and glucocorticoids. Neuron 11 371-386 (1993).

5 Zhang, V., O'Sullivan, M., Hussain, H., et al. Molecular cloning, functional expression, and selective regulation of ovine prostaglandin H synthase-2. Biochem Biophys Res Commun 227 499-506 (1996).

Synonyms
  • Mouse macrophage microsomes protein
Formulation 500 µg mouse macrophage microsomal protein in 500 µl sample buffer
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Hla, T., and Neilson, K. Human cyclooxygenase-2 cDNA. Proc Natl Acad Sci USA 89 7384-7388 (1992).

Kujubu, D.A., Fletcher, B.S., Varnum, B.C., et al. TIS10, a phorbol ester tumor promoter-inducible mRNA from Swiss 3T3 cells, encodes a novel prostaglandin synthase/cyclooxygenase homologue. J Biol Chem 266 12866-12872 (1991).

Yamagata, K., Andreasson, K.I., Kaufmann, W.E., et al. Expression of a mitogen-inducible cyclooxygenase in brain neurons: Regulation by synaptic activity and glucocorticoids. Neuron 11 371-386 (1993).

Zhang, V., O'Sullivan, M., Hussain, H., et al. Molecular cloning, functional expression, and selective regulation of ovine prostaglandin H synthase-2. Biochem Biophys Res Commun 227 499-506 (1996).

Smith, W.L., Garavito, R.M., and DeWitt, D.L. Prostaglandin endoperoxide H synthases (cyclooxygenases)-1 and -2. J Biol Chem 271 33157-33160 (1996).

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Pricing updated 2012-02-12. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

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