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Nod2 Monoclonal Antibody (Clone 2D9)

Cayman Chemical Item Number 10004942

Nod2 Monoclonal Antibody (Clone 2D9)

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Description

Antigen: human recombinant Nod2 amino acids 28-301 · Clone designation: 2D9 · Host: mouse · Application(s): WB and IHC; other applications not tested · Nod2 is a member of the apoptosis regulating protein family that has caspase recruitment-domains (CARDs) and also includes Apaf-1 and Nod1.1 Nod2 contains two N-terminal CARDs, a nucleotide binding domain (NBD), and multiple C-terminal leucine-rich repeats (LRRs). Nod2 is expressed in monocytes whereas Nod1 (CARD4) is expressed in multiple tissues.1,2,3,4 Both Nod1 and Nod2 act as intracellular receptors for bacterial lipopolysaccharides, activate NF-kB, and contribute to inflammatory bowel disease.3,5,6,7 A frameshift mutation in the NOD2 gene is associated with susceptibility to Crohn’s disease, possibly by causing truncation of the tenth LRR.8,9 Cayman Chemical’s NOD2 Monoclonal Antibody can be used for western blot and immunohistochemical analysis of Nod2 on samples of human origin. The antibody will also recognize three Nod2 polymorphisms: L1007finsC, G908R, and R702W.2

1 Ogura, Y., Inohara, N., Benito, A., et al. Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-κB. J Biol Chem 276 4812-4818 (2001).

2 Ogura, Y., Lala, S., Xin, W., et al. Expression of NOD2 in Paneth cells: A possible link to Crohn’s ileitis. Gut 52 1591-1597 (2003).

3 Bertin, J., Nir, W., Fischer, C.M., et al. Human CARD4 protein is a novel CED-4/Apaf-1 cell death family member that activates NF-κB. J Biol Chem 274 12955-12958 (1999).

4 Gutierrez, O., Pipaon, C., Inohara, N., et al. Induction of Nod2 in myelomonocytic and intestinal epithelial cells via nuclear factor-κB activation. J Biol Chem 277(44) 41701-41705 (2002).

5 Inohara, N., Koseki, T., del Peso, L., et al. Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-κB. J Biol Chem 274 14560-14567 (1999).

6 Inohara, N., Ogura, Y., Chen, F.F., et al. Human NOD1 confers responsiveness to bacterial lipopolysaccharides. J Biol Chem 276 2551-2554 (2001).

7 Inohara, N., Koseki, T., Lin, J., et al. An induced proximity model for NF-κB activation in the Nod1/RICK and RIP signaling pathways. J Biol Chem 275 27823-27831 (2000).

8 Ogura, Y., Bonen, D.K., Inohara, N., et al. A frameshift mutation in NOD2 associated with susceptibility to Crohn’s disease. Nature 411 603-606 (2001).

9 Hugot, J., Chamaillard, M., Zouali, H., et al. Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn’s disease. Nature 411 599-603 (2001).

Formulation 200 µg protein-a purified IgG1.kappa. in 200 µl PBS, pH 7.4, containing 0.02% sodium azide
Stability 1 year
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Human Nod2 +

Background Reading

Ogura, Y., Inohara, N., Benito, A., et al. Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-κB. J Biol Chem 276 4812-4818 (2001).

Hugot, J., Chamaillard, M., Zouali, H., et al. Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn’s disease. Nature 411 599-603 (2001).

Ogura, Y., Bonen, D.K., Inohara, N., et al. A frameshift mutation in NOD2 associated with susceptibility to Crohn’s disease. Nature 411 603-606 (2001).

Bertin, J., Nir, W., Fischer, C.M., et al. Human CARD4 protein is a novel CED-4/Apaf-1 cell death family member that activates NF-κB. J Biol Chem 274 12955-12958 (1999).

Inohara, N., Koseki, T., del Peso, L., et al. Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-κB. J Biol Chem 274 14560-14567 (1999).

Inohara, N., Ogura, Y., Chen, F.F., et al. Human NOD1 confers responsiveness to bacterial lipopolysaccharides. J Biol Chem 276 2551-2554 (2001).

Inohara, N., Koseki, T., Lin, J., et al. An induced proximity model for NF-κB activation in the Nod1/RICK and RIP signaling pathways. J Biol Chem 275 27823-27831 (2000).

Gutierrez, O., Pipaon, C., Inohara, N., et al. Induction of Nod2 in myelomonocytic and intestinal epithelial cells via nuclear factor-κB activation. J Biol Chem 277(44) 41701-41705 (2002).

Ogura, Y., Lala, S., Xin, W., et al. Expression of NOD2 in Paneth cells: A possible link to Crohn’s ileitis. Gut 52 1591-1597 (2003).

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1 ea $229.00 $0.00
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This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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