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PEPCK Polyclonal Antibody

Cayman Chemical Item Number 10004943

Phosphoenolpyruvate carboxykinase; PEPCK-c; Pck1

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Description

Antigen: mouse and rat PEPCK protein amino acids 5-17 (LHNGLDFSAKVIQ) · Host: rabbit · Application(s): WB; other applications not tested · Phosphoenolpyruvate carboxykinase (PEPCK) is a hormonally regulated enzyme responsible for the first committed step in gluconeogenesis, catalyzing the conversion of oxaloacetate to phosphoenolpyruvate.1,2 PEPCK mRNA is most abundant in the liver, kidney cortex, and white adipose tissue but is also found in brown fat, the mammary gland during lactation, and the small intestine.2 Both non-insulin-dependent diabetes mellitus and streptozotocin-induced diabetes result in elevated PEPCK activity, protein, and mRNA.1,3 Two isozymes of PEPCK have been identified, cytosolic and mitochondrial.4 Cayman’s PEPCK polyclonal antibody was generated against murine cytosolic PEPCK and detects the protein at 63 kDa by western blotting in rat and murine samples.

1 Friedman, J.E., Yun, J.S., Patel, Y.M., et al. Glucocorticoids regulate the induction of phosphoenolpyruvate carboxykinase (GTP) gene transcription during diabetes. J Biol Chem 268(17) 12952-12957 (1993).

2 Rajas, F., Croset, M., Zitoun, C., et al. Induction of PEPCK gene expression in insulinopenia in rat small intestine. Diabetes 49 1165-1168 (2000).

3 Veneziale, C.M., Donofrio, J.C., and Nishimura, H. The concentration of P-enolpyruvate carboxykinase protein in murine tissues in diabetes of chemical and genetic origin. J Biol Chem 258(23) 14257-14262 (1983).

4 Weldon, S.L., Rando, A., Matathias, A.S., et al. Mitochondrial phosphoenolpyruvate carboxykinase from the chicken. Comparison of the cDNA and protein sequences with the cytosolic isozyme. J Biol Chem 265(13) 7308-7317 (1990).

Synonyms
  • Phosphoenolpyruvate carboxykinase
  • PEPCK-c
  • Pck1
Formulation Peptide affinity-purified IgG
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Mouse PEPCK +
Rat PEPCK +

Background Reading

Friedman, J.E., Yun, J.S., Patel, Y.M., et al. Glucocorticoids regulate the induction of phosphoenolpyruvate carboxykinase (GTP) gene transcription during diabetes. J Biol Chem 268(17) 12952-12957 (1993).

Weldon, S.L., Rando, A., Matathias, A.S., et al. Mitochondrial phosphoenolpyruvate carboxykinase from the chicken. Comparison of the cDNA and protein sequences with the cytosolic isozyme. J Biol Chem 265(13) 7308-7317 (1990).

Veneziale, C.M., Donofrio, J.C., and Nishimura, H. The concentration of P-enolpyruvate carboxykinase protein in murine tissues in diabetes of chemical and genetic origin. J Biol Chem 258(23) 14257-14262 (1983).

Rajas, F., Croset, M., Zitoun, C., et al. Induction of PEPCK gene expression in insulinopenia in rat small intestine. Diabetes 49 1165-1168 (2000).

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Size Price Quantity Subtotal
500 µl $229.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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