Antigen: mouse and rat PEPCK protein amino acids 5-17 (LHNGLDFSAKVIQ) · Host: rabbit · Application(s): WB; other applications not tested · Phosphoenolpyruvate carboxykinase (PEPCK) is a hormonally regulated enzyme responsible for the first committed step in gluconeogenesis, catalyzing the conversion of oxaloacetate to phosphoenolpyruvate.^1,2 PEPCK mRNA is most abundant in the liver, kidney cortex, and white adipose tissue but is also found in brown fat, the mammary gland during lactation, and the small intestine.² Both non-insulin-dependent diabetes mellitus and streptozotocin-induced diabetes result in elevated PEPCK activity, protein, and mRNA.^1,3 Two isozymes of PEPCK have been identified, cytosolic and mitochondrial.⁴ Cayman’s PEPCK polyclonal antibody was generated against murine cytosolic PEPCK and detects the protein at 63 kDa by western blotting in rat and murine samples.

1 Friedman, J.E., Yun, J.S., Patel, Y.M., et al. Glucocorticoids regulate the induction of phosphoenolpyruvate carboxykinase (GTP) gene transcription during diabetes. J Biol Chem 268(17) 12952-12957 (1993).

2 Rajas, F., Croset, M., Zitoun, C., et al. Induction of PEPCK gene expression in insulinopenia in rat small intestine. Diabetes 49 1165-1168 (2000).

3 Veneziale, C.M., Donofrio, J.C., and Nishimura, H. The concentration of P-enolpyruvate carboxykinase protein in murine tissues in diabetes of chemical and genetic origin. J Biol Chem 258(23) 14257-14262 (1983).

4 Weldon, S.L., Rando, A., Matathias, A.S., et al. Mitochondrial phosphoenolpyruvate carboxykinase from the chicken. Comparison of the cDNA and protein sequences with the cytosolic isozyme. J Biol Chem 265(13) 7308-7317 (1990).

Friedman, J.E., Yun, J.S., Patel, Y.M., et al. Glucocorticoids regulate the induction of phosphoenolpyruvate carboxykinase (GTP) gene transcription during diabetes. J Biol Chem 268(17) 12952-12957 (1993).

Weldon, S.L., Rando, A., Matathias, A.S., et al. Mitochondrial phosphoenolpyruvate carboxykinase from the chicken. Comparison of the cDNA and protein sequences with the cytosolic isozyme. J Biol Chem 265(13) 7308-7317 (1990).

Veneziale, C.M., Donofrio, J.C., and Nishimura, H. The concentration of P-enolpyruvate carboxykinase protein in murine tissues in diabetes of chemical and genetic origin. J Biol Chem 258(23) 14257-14262 (1983).

Rajas, F., Croset, M., Zitoun, C., et al. Induction of PEPCK gene expression in insulinopenia in rat small intestine. Diabetes 49 1165-1168 (2000).

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