See personalized New Product recommendations! Get personalized New Product recommendations! Register or Login for personalized New Product recommendations!

Holiday Notification: Cayman Chemical will be closed Monday, May 28, 2012, in observance of the Memorial Day holiday. More…
Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

Prostaglandin Metabolite HPLC Mixture

Cayman Chemical Item Number 10005

PG Metabolite HPLC Mixture

See more

Description

This mixture contains the primary metabolites of prostaglandins D2, E2, and F2α. Contents: 13,14-dihydro-15-keto Prostaglandin D2, 13,14-dihydro-15-keto Prostaglandin E2, 11β-Prostaglandin F2α, 13,14-dihydro-15-keto Prostaglandin F2α, and Prostaglandin F2β (100 µg each). In primates, the lung is an active site of prostaglandin (PG) inactivation via 15-hydroxy PG dehydrogenase (15-hydroxy PGDH).1 E and F-series PGs are good substrates for 15-hydroxy PGDH, which oxidizes the C-15 hydroxyl group to give the corresponding 15-keto compounds.2 This oxidation if followed rapidly by Δ13-reductase removal of the 13,14-double bond, to give 13,14-dihydro-15-keto PG metabolites.3 While the primary PGs are often undetectable in plasma due to their rapid inactivation, the 13,14-dihydro-15-keto metabolites can be present at concentrations of several hundred pg/ml.4 In addition, 9- and 11-keto PG-reductases have been reported, especially in the case of PGD2, which produce F-series PG metabolites with a β-hydroxyl group on the ring.5 These metabolites span a wide polarity range and separate well on reversed phase HPLC.

1 Hansen, H.S. 15-Hydroxyprostaglandin dehydrogenase. A review. Prostaglandins 12 647-679 (1976).

2 Sun, F.F., Armour, S.B., Bockstanz, V.R., et al. Studies on 15-hydroxyprostaglandin dehydrogenase from monkey lung. Adv Prostaglandin Thromboxane Res 1 163-169 (1976).

3 Basu, S., and Stjernschantz, J. Δ13-reductase dependent metabolism of prostaglandins in the mammalian brain and eye. Prostaglandins Leukot Essent Fatty Acids 57 305-310 (1997).

4 Granström, E., Hamberg, M., Hansson, G., et al. Chemical instability of 15-keto-13,14-dihydro-PGE2: The reason for low assay reliability. Prostaglandins 19 933-945 (1980).

5 Ellis, C.K., Smigel, M.D., Oates, J.A., et al. Metabolism of prostaglandin D2 in the monkey. J Biol Chem 254 4152-4163 (1979).

Synonyms
  • PG Metabolite HPLC Mixture
Formulation A solution in methyl acetate
Purity >98% for each compound
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Sun, F.F., Armour, S.B., Bockstanz, V.R., et al. Studies on 15-hydroxyprostaglandin dehydrogenase from monkey lung. Adv Prostaglandin Thromboxane Res 1 163-169 (1976).

Granström, E., Hamberg, M., Hansson, G., et al. Chemical instability of 15-keto-13,14-dihydro-PGE2: The reason for low assay reliability. Prostaglandins 19 933-945 (1980).

Hansen, H.S. 15-Hydroxyprostaglandin dehydrogenase. A review. Prostaglandins 12 647-679 (1976).

Ellis, C.K., Smigel, M.D., Oates, J.A., et al. Metabolism of prostaglandin D2 in the monkey. J Biol Chem 254 4152-4163 (1979).

Basu, S., and Stjernschantz, J. Δ13-reductase dependent metabolism of prostaglandins in the mammalian brain and eye. Prostaglandins Leukot Essent Fatty Acids 57 305-310 (1997).

Show all 5 Hide all but first 3
Size Price Quantity Subtotal
1 ea $72.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

Related Products

11β-Prostaglandin F2α
13,14-dihydro-15-keto Prostaglandin D2
13,14-dihydro-15-keto Prostaglandin D2 Lipid Maps MS Standard
13,14-dihydro-15-keto Prostaglandin E2
13,14-dihydro-15-keto Prostaglandin E2 Lipid Maps MS Standard
13,14-dihydro-15-keto Prostaglandin F2α
Cyclopentenone Prostaglandin HPLC Mixture
Primary Eicosanoid HPLC Mixture
Prostaglandin F2β
Prostaglandin HPLC Mixture
Vasoactive Eicosanoid HPLC Mixture
Show all 11 Hide all but first 3

Downloads

Batch-specific Information

Login to access batch-specific information

Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

Other Resources
Management Team Company Profile Company History ChemAssistant Tools FAQs Cayman Europe Cayman Pharma CaBRI Cayman Gear Press Releases Illustrations and Charts Key Research Area Posters Article Library Analysis Tools Conference Schedule Order Terms Browser Recommendation Privacy Statement Site Map

Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640

Copyright 2012 Cayman Chemical Company

Lost password?