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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Prostaglandin Metabolite HPLC Mixture

Cayman Chemical Item Number 10005

PG Metabolite HPLC Mixture

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Description

This mixture contains the primary metabolites of prostaglandins D2, E2, and F2α. Contents: 13,14-dihydro-15-keto Prostaglandin D2, 13,14-dihydro-15-keto Prostaglandin E2, 11β-Prostaglandin F2α, 13,14-dihydro-15-keto Prostaglandin F2α, and Prostaglandin F2β (100 µg each). In primates, the lung is an active site of prostaglandin (PG) inactivation via 15-hydroxy PG dehydrogenase (15-hydroxy PGDH).1 E and F-series PGs are good substrates for 15-hydroxy PGDH, which oxidizes the C-15 hydroxyl group to give the corresponding 15-keto compounds.2 This oxidation if followed rapidly by Δ13-reductase removal of the 13,14-double bond, to give 13,14-dihydro-15-keto PG metabolites.3 While the primary PGs are often undetectable in plasma due to their rapid inactivation, the 13,14-dihydro-15-keto metabolites can be present at concentrations of several hundred pg/ml.4 In addition, 9- and 11-keto PG-reductases have been reported, especially in the case of PGD2, which produce F-series PG metabolites with a β-hydroxyl group on the ring.5 These metabolites span a wide polarity range and separate well on reversed phase HPLC.

1 Hansen, H.S. 15-Hydroxyprostaglandin dehydrogenase. A review. Prostaglandins 12 647-679 (1976).

2 Sun, F.F., Armour, S.B., Bockstanz, V.R., et al. Studies on 15-hydroxyprostaglandin dehydrogenase from monkey lung. Adv Prostaglandin Thromboxane Res 1 163-169 (1976).

3 Basu, S., and Stjernschantz, J. Δ13-reductase dependent metabolism of prostaglandins in the mammalian brain and eye. Prostaglandins Leukot Essent Fatty Acids 57 305-310 (1997).

4 Granström, E., Hamberg, M., Hansson, G., et al. Chemical instability of 15-keto-13,14-dihydro-PGE2: The reason for low assay reliability. Prostaglandins 19 933-945 (1980).

5 Ellis, C.K., Smigel, M.D., Oates, J.A., et al. Metabolism of prostaglandin D2 in the monkey. J Biol Chem 254 4152-4163 (1979).

Synonyms
  • PG Metabolite HPLC Mixture
Formulation A solution in methyl acetate
Purity >98% for each compound
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Sun, F.F., Armour, S.B., Bockstanz, V.R., et al. Studies on 15-hydroxyprostaglandin dehydrogenase from monkey lung. Adv Prostaglandin Thromboxane Res 1 163-169 (1976).

Granström, E., Hamberg, M., Hansson, G., et al. Chemical instability of 15-keto-13,14-dihydro-PGE2: The reason for low assay reliability. Prostaglandins 19 933-945 (1980).

Hansen, H.S. 15-Hydroxyprostaglandin dehydrogenase. A review. Prostaglandins 12 647-679 (1976).

Ellis, C.K., Smigel, M.D., Oates, J.A., et al. Metabolism of prostaglandin D2 in the monkey. J Biol Chem 254 4152-4163 (1979).

Basu, S., and Stjernschantz, J. Δ13-reductase dependent metabolism of prostaglandins in the mammalian brain and eye. Prostaglandins Leukot Essent Fatty Acids 57 305-310 (1997).

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Pricing updated 2012-02-12. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

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