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Protein Carbonyl Assay KitCayman Chemical Item Number 10005020
Description
Cayman Chemical’s Protein Carbonyl Assay Kit is a convenient colorimetric assay for the measurement of oxidized proteins. Protein samples are derivatized by making use of the reaction between 2,4-
Background ReadingHalliwell, B. Oxidative stress, nutrition and health. Experimental strategies for optimization of nutritional antioxidant intake in humans. Free Radic Res 25 57-74 (1996). Stadtman, E.R., and Oliver, C.N. Metal- Levine, R.L., Williams, J.A., Stadtman, E.R., et al. Carbonyl assays for determination of oxidatively modified proteins. Methods Enzymol 233 346-357 (1994). Reznick, A.Z., and Packer, L. Oxidative damage to proteins: Spectrophotometric method for carbonyl assay. Methods Enzymol 233 357-363 (1994). Zusterzeel, P.L.M., Mulder, T.P.J., Peters, W.H.M., et al. Plasma protein carbonyls in nonpregnant, healthy pregnant and preeclamptic womern. Free Radic Res 33 471-476 (2000). Reznick, A.Z., Cross, C.E., Hu, M., et al. Modification of plasma proteins by cigarette smoke as measured by protein carbonyl formation. Biochem J 286 607-611 (1992). Show all 6 Hide all but first 3 |
This product is also available to buy in bulk quantities. Please contact our Sales Department for a quote or to purchase. Pricing updated 2012-02-10. Prices are subject to change without notice. To ask for assistance with one of our products please contact a Technical Support Scientist. Warning This product is not for human or veterinary use. You may be eligible to receive a free sample Protein Carbonyl Assay Kit under the Cayman Challenge program. Let Cayman analyze your samples for you. See EIA Service for details and availability. Related ProductsShow all 19 Hide all but first 3Downloads
Batch-specific InformationLogin to access batch-specific information FAQsFor the Protein Carbonyl Assay Kit are there recommendations for use of samples dissolved in buffer that is 30 mM Tris-HCl, 7 M Urea, 2 M Thiourea, 4% CHAPS, Protease inhibitor cocktail, pH 8.5? The buffer you are using is designed to solubilize proteins. It is possible that the protein in your samples will have a difficult time precipitating in the assay. The carbonyl groups in urea and CHAPS should not react with the DNPH in the assay, but it is unknown whether or not these components will interfere (as other substances that contain no carbonyl groups can still interfere, such as Triton X-100). The assay may suit your needs but we cannot guarantee this. Show answerFor the Protein Carbonyl Assay Kit You indicate the following equation; Protein Carbonyl(nmol/ml)=[(CA)/(*0.011uM^-1)](500ul/200ul) Does an actual calculation simply divide CA by 0.011? What do you mean "(500 µl/200 µl)" ? Dividing CA by 0.011µM^-1 will give the concentration of protein carbonyl for the 500µl guanidine hydrochloride solution. Performing the 500µl/200µl will give the concentration of protein carbonyl in the original 200µl sample. If a customer only used a 100µl sample, for example, the equation would be adjusted to 500µl/100µl. This term in the equation accounts for sample dilution. Show answer |
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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins. |
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Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA
Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640
Copyright 2012 Cayman Chemical Company
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