Antigen: human PTEN amino acids 254-270 (KVEFFHKQNKMLKKDKM) · Host: rabbit · Application(s): WB and IHC (formalin-fixed paraffin-embedded sections); other applications not tested · PTEN (phosphatase and tensin homology on chromosome 10) dephosphorylates proteins and lipids such as AKT and phosphatidylinositol 3,4,5-trisphosphate (PIP₃) and therefore functions as a key regulatory enzyme in a central signal transduction pathway. It also interacts with proteins Shc and FAK to promote apoptosis when necessary and to regulate cell division and migration.¹ PTEN is considered a tumor suppressor as loss-of-function mutations in PTEN often result in human cancers including melanoma and prostate carcinoma.^2,3 PTEN is expressed in almost all tissues of the body as a 403 amino acid protein with an estimated molecular weight of 47 kDa.⁴ Cayman’s PTEN polyclonal antibody can be used for western blot (WB) and immunohistochemical analysis (paraffin-embedded tissue sections) on samples from human, chimpanzee, mouse, rat, and dog. It detects the protein from many tissues, as well as from cultured cells such as A431 and HEK293, at 48-50 kDa by WB.

1 Di Cristofano, A., and Pandolfi, P.P. The multiple roles of PTEN in tumor suppression. Cell 100 387-390 (2000).

2 Vivanco, I., and Sawyers, C.L. The phosphatidylinositol 3-kinase-AKT pathway in human cancer. Nat Rev Cancer 2 489-501 (2002).

3 Dahia, P.L.M. PTEN, a unique tumor suppressor gene. Endocr Relat Cancer 7 115-129 (2000).

4 Steck, P.A., Pershouse, M.A., Jasser, S.A., et al. Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers. Nat Genet 15 356-362 (1997).

Steck, P.A., Pershouse, M.A., Jasser, S.A., et al. Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers. Nat Genet 15 356-362 (1997).

Vivanco, I., and Sawyers, C.L. The phosphatidylinositol 3-kinase-AKT pathway in human cancer. Nat Rev Cancer 2 489-501 (2002).

Dahia, P.L.M. PTEN, a unique tumor suppressor gene. Endocr Relat Cancer 7 115-129 (2000).

Di Cristofano, A., and Pandolfi, P.P. The multiple roles of PTEN in tumor suppression. Cell 100 387-390 (2000).

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