Cayman Chemical | U19 Polyclonal Antibody • ELL-Associated Factor 2; EAF2

U19 Polyclonal Antibody

Cayman Chemical Item Number 10005190

ELL-Associated Factor 2; EAF2

U19 Polyclonal Antibody

Synonyms

ELL-Associated Factor 2
EAF2

Formulation

150 µg peptide affinity-purified IgG in 300 µl TBS, pH 7.4, containing 50% glycerol, 0.1% BSA, and 0.02% sodium azide

Stability

1 year

Storage

-20°C

Shipping

WET ICE in continental US; may vary elsewhere

Description

Antigen: human U19 amino acids 5-16 (AGFSHLDRRERV) · Host: rabbit · Application(s): WB and IHC (formalin-fixed paraffin-embedded sections) · U19 is a testosterone-regulated apoptosis inducer with tumor suppressive activity.¹ In vivo, overexpression of U19 induces massive apoptosis and inhibits prostate tumor growth.¹ It was first identified as ELL-associated factor 2 (EAF2), which shares high homology with EAF1 that also interacts with ELL, and may play a role in leukemogenesis.² U19/EAF2 mRNA is widely expressed in various tissues, with the most abundant expression in the prostate, kidney, bone marrow, and lymph nodes.¹ Cayman Chemical’s U19 Polyclonal Antibody can be used for western blot and immunohistochemical (formalin-fixed paraffin-embedded sections) analysis for U19 on samples from human, mouse, and rat. Other applications for use of this antibody have not yet been tested. The antibody detects a 43 kDa endogenous protein by western blot in human, murine, and rat samples, which is consistent with published data for U19.²

1 Xiao, W., Zhang, Q., Jiang, F., et al. Suppression of prostate tumor growth by U19, a novel testosterone-regulated apoptosis inducer. Cancer Res 63 4698-4704 (2003).

2 Simone, F., Luo, R.T., Polak, P.E., et al. ELL-associated factor 2 (EAF2), a functional homolog of EAF1 with alternative ELL binding properties. Blood 101(6) 2355-2362 (2003).

Simone, F., Luo, R.T., Polak, P.E., et al. ELL-associated factor 2 (EAF2), a functional homolog of EAF1 with alternative ELL binding properties. Blood 101(6) 2355-2362 (2003).

Xiao, W., Zhang, Q., Jiang, F., et al. Suppression of prostate tumor growth by U19, a novel testosterone-regulated apoptosis inducer. Cancer Res 63 4698-4704 (2003).