Antigen: human EAF2 amino acids 5-16 (AGFSHLDRRERV) · Host: rabbit · Application(s): WB and IHC (formalin-fixed paraffin-embedded sections) · EAF2 is a testosterone-regulated apoptosis inducer with tumor suppressive activity.¹ In vivo, overexpression of EAF2 induces massive apoptosis and inhibits prostate tumor growth.¹ It was first identified as ELL-associated factor 2, which shares high homology with EAF1 that also interacts with ELL, and may play a role in leukemogenesis.² EAF2 mRNA is widely expressed in various tissues, with the most abundant expression in the prostate, kidney, bone marrow, and lymph nodes.¹ Cayman Chemical’s EAF2 Polyclonal Antibody can be used for western blot and immunohistochemical (formalin-fixed paraffin-embedded sections) analysis for EAF2 on samples from human, mouse, and rat. Other applications for use of this antibody have not yet been tested. The antibody detects a 43 kDa endogenous protein by western blot in human, murine, and rat samples, which is consistent with published data for EAF2.²

1 Xiao, W., Zhang, Q., Jiang, F., et al. Suppression of prostate tumor growth by U19, a novel testosterone-regulated apoptosis inducer. Cancer Res 63 4698-4704 (2003).

2 Simone, F., Luo, R.T., Polak, P.E., et al. ELL-associated factor 2 (EAF2), a functional homolog of EAF1 with alternative ELL binding properties. Blood 101(6) 2355-2362 (2003).

Simone, F., Luo, R.T., Polak, P.E., et al. ELL-associated factor 2 (EAF2), a functional homolog of EAF1 with alternative ELL binding properties. Blood 101(6) 2355-2362 (2003).

Xiao, W., Zhang, Q., Jiang, F., et al. Suppression of prostate tumor growth by U19, a novel testosterone-regulated apoptosis inducer. Cancer Res 63 4698-4704 (2003).