Antigen: human S1P₁ amino acids 241-253 (ISKASRSSEKSLA) · Host: rabbit · Application(s): WB, IHC (formalin-fixed paraffin-embedded sections), and ICC; other applications not tested · Sphingosine-1-phosphate (S1P) exerts its activity by binding to five distinct G-protein-coupled receptors, S1P₁/EDG-1, S1P₂/EDG-5, S1P₃/EDG-3, S1P₄/EDG-6, and S1P₅/EDG-8.^1,2 S1P₁ primarily couples with pertussis toxin-sensitive G_i/o proteins to mediate S1P-induced cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis.^1,2,3 Expression of S1P₁ is abundant in embryological vasculature and is ubiquitously expressed in adult cells suggesting diverse physiological functions of this receptor.² The human and mouse S1P₁ receptors have 382 amino acids with an estimated molecular weight of 43 kDa. Glycosylation at the N-terminal extracellular domain may cause the protein to migrate at a higher position in SDS-PAGE.⁴ Cayman’s S1P₁ polyclonal antibody detects the receptor at 47 kDa by western blot analysis. The antibody can also be used for immunocytochemistry and immunohistochemistry to study expression patterns of this protein.

1 Takuwa, Y., Takuwa, N., and Sugimoto, N. The Edg family G protein-coupled receptors for lysophospholipids: Their signaling properties and biological activities. J Biochem 131 767-771 (2002).

2 Ishii, I., Fukushima, N., Ye, X., et al. Lysophospholipid receptors: Signaling and biology. Annu Rev Biochem 73 321-354 (2004).

3 Kluk, M.J., and Hla, T. Signaling of sphingosine-1-phosphate via the S1P/EDG-family of G-protein-coupled receptors. Biochim Biophys Acta 1582 72-80 (2002).

4 Kohno, T., Wada, A., and Igarashi, Y. N-glycans of sphingosine 1-phosphate receptor Edg-1 regulate ligand-induced receptor internalization. FASEB J 16 983-992 (2002).

Takuwa, Y., Takuwa, N., and Sugimoto, N. The Edg family G protein-coupled receptors for lysophospholipids: Their signaling properties and biological activities. J Biochem 131 767-771 (2002).

Kluk, M.J., and Hla, T. Signaling of sphingosine-1-phosphate via the S1P/EDG-family of G-protein-coupled receptors. Biochim Biophys Acta 1582 72-80 (2002).

Kohno, T., Wada, A., and Igarashi, Y. N-glycans of sphingosine 1-phosphate receptor Edg-1 regulate ligand-induced receptor internalization. FASEB J 16 983-992 (2002).

Ishii, I., Fukushima, N., Ye, X., et al. Lysophospholipid receptors: Signaling and biology. Annu Rev Biochem 73 321-354 (2004).

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