Serine Palmitoyltransferase Polyclonal Antibody

Cayman Chemical Item Number 10005260

Serine-palmitoyl-CoA transferase, long chain base subunit 2; SPT

Description

Antigen: human serine palmitoyltransferase subunit SPT2 amino acids 548-562 · Host: rabbit · Application(s): WB and IHC (formalin-fixed paraffin-embedded sections); other applications not tested · Sphingolipids play essential roles in various cellular events, including proliferation, differentiation, senescence, apoptosis, and inflammatory responses.¹ Serine palmitoyltransferase (SPT) is the initial and rate-limiting enzyme in the de novo sphingolipid biosynthesis, and thus regulates the level of sphingolipids in cells.² Immunohistochemical study revealed widespread distribution of the enzyme with most strong expression in brain and digestive tract.³ Two subunits, SPT1 and SPT2 at a stoichiometry of 1:1, are involved in the enzymatic activity of SPT.⁴ Cayman Chemical’s serine palmitoyltransferase polyclonal antibody recognizes SPT2, the long chain subunit of the enzyme. The antibody stains mainly cell nuclei and occasionally both cell nuclei and cytoplasm in formalin-fixed, paraffin-embedded rat brain tissue. The nuclear localization of SPT2 may suggest that SPT2 associates with another nuclear protein or is modified and transported to the nucleus.²

1 Mathias, S., Peña, L.A., and Kolesnick, R.N. Signal transduction of stress via ceramide. Biochem J 335 465-480 (1998).

2 Carton, J.M., Uhlinger, D.J., Batheja, A.D., et al. Enhanced serine palmitoyltransferase expression in proliferating fiboblasts, transformed cell lines, and human tumors. J Histochem Cytochem 51(6) 715-726 (2003).

3 Batheja, A.D., Uhlinger, D.J., Carton, J.M., et al. Characterization of serine palmitoyltransferase in normal human tissues. J Histochem Cytochem 51(5) 687-696 (2003).

4 Hanada, K., Hara, T., and Nishijima, M. Purification of the serine palmitoyltransferase complex responsible for sphingoid base synthesis by using affinity peptide chromatography techniques. J Biol Chem 275(12) 8409-8415 (2000).

Synonyms

Serine-palmitoyl-CoA transferase, long chain base subunit 2
SPT

Formulation

Peptide affinity-purified IgG

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Specificity

Human serine palmitoyltransferase	+
Mouse serine palmitoyltransferase	+
Rat serine palmitoyltransferase	+
Ovine serine palmitoyltransferase	+
Porcine serine palmitoyltransferase	+
Bovine serine palmitoyltransferase	+
African green monkey serine palmitoyltransferase	+

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Background Reading

Batheja, A.D., Uhlinger, D.J., Carton, J.M., et al. Characterization of serine palmitoyltransferase in normal human tissues. J Histochem Cytochem 51(5) 687-696 (2003).

Hanada, K., Hara, T., and Nishijima, M. Purification of the serine palmitoyltransferase complex responsible for sphingoid base synthesis by using affinity peptide chromatography techniques. J Biol Chem 275(12) 8409-8415 (2000).

Carton, J.M., Uhlinger, D.J., Batheja, A.D., et al. Enhanced serine palmitoyltransferase expression in proliferating fiboblasts, transformed cell lines, and human tumors. J Histochem Cytochem 51(6) 715-726 (2003).

Mathias, S., Peña, L.A., and Kolesnick, R.N. Signal transduction of stress via ceramide. Biochem J 335 465-480 (1998).

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Subtotal

500 µl

$270.00

$0.00

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