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Antigen:
human lysophosphatidic acid 1 (LPA1) amino acids 342-359 (DRSASSLNHTILAGVHSN)
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Host:
rabbit
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Application(s):
WB and ICC; other applications not tested
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LPA1 (also known as EDG-2) is one of four known LPA receptors (LPA1-4).1 LPA1-3 are part a family of G protein-coupled receptors that share identity to the sphingosine-1-phosphate receptors (S1P1-5).2 LPA1 couples with three types of G proteins, Gi/o, Gq, and G12/13 to induce a range of cellular responses including activation of phospholipase C, multiple kinases, the serum response element and cell proliferation.3,4 LPA1 mRNA is detected a wide range of tissues and cells including brain, heart, small intestine, spleen, testis, kidney, HeLa cells, and A549 cells.5,6 Murine and human LPA1 have 364 amino acids with an estimated molecular weight of 41 kDa.4 Cayman’s LPA1 polyclonal antibody detects the protein from several species from 43-50 kDa, suggesting heterogeneous LPA1 post-translational modifications in distinct cells.
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1
Noguchi, K., Ishii, S., Shimizu, T. Identification of p2y9/GPR23 as a novel G protein-coupled receptor for lysophosphatidic acid, structurally distant from the Edg family. J Biol Chem 278(28) 25600-25606 (2003).
2
Chun, J., Goetzl, E.J., Hla, T., et al. International union of pharmacology. XXXIV. Lysophospholipid receptor nomenclature. Pharmacol Rev 54 265-269 (2002).
3
Fukushima, N., Chun, J. The LPA receptors. Prostaglandins and Other Lipid Mediators 64 21-32 (2001).
4
Ishii, I., Fukushima, N., Ye, X., et al. Lysophospholipid receptors: Signaling and biology. Annu Rev Biochem 73 321-354 (2004).
5
Contos, J.J.A., Chun, J. The mouse lpA3/Edg7 lysophosphatidic acid receptor gene: genomic structure, chromosomal localization, and expression pattern. Gene 267 243-253 (2001).
6
An, S., Bleu, T., Hallmark, O.G., et al. Characterization of a novel subtype of human G protein-coupled receptor for lysophosphatidic acid. J Biol Chem 273(14) 7906-7910 (1998).
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