Antigen: human lysophosphatidic acid 1 (LPA₁) amino acids 342-359 (DRSASSLNHTILAGVHSN) · Host: rabbit · Application(s): WB and ICC; other applications not tested · LPA₁ (also known as EDG-2) is one of four known LPA receptors (LPA_1-4).¹ LPA_1-3 are part a family of G protein-coupled receptors that share identity to the sphingosine-1-phosphate receptors (S1P_1-5).² LPA₁ couples with three types of G proteins, G_i/o, G_q, and G_12/13 to induce a range of cellular responses including activation of phospholipase C, multiple kinases, the serum response element and cell proliferation.^3,4 LPA₁ mRNA is detected a wide range of tissues and cells including brain, heart, small intestine, spleen, testis, kidney, HeLa cells, and A549 cells.^5,6 Murine and human LPA₁ have 364 amino acids with an estimated molecular weight of 41 kDa.⁴ Cayman’s LPA₁ polyclonal antibody detects the protein from several species from 43-50 kDa, suggesting heterogeneous LPA₁ post-translational modifications in distinct cells.

1 Noguchi, K., Ishii, S., and Shimizu, T. Identification of p2y9/GPR23 as a novel G protein-coupled receptor for lysophosphatidic acid, structurally distant from the Edg family. J Biol Chem 278(28) 25600-25606 (2003).

2 Chun, J., Goetzl, E.J., Hla, T., et al. International union of pharmacology. XXXIV. Lysophospholipid receptor nomenclature. Pharmacol Rev 54 265-269 (2002).

3 Fukushima, N., and Chun, J. The LPA receptors. Prostaglandins Other Lipid Mediat 64 21-32 (2001).

4 Ishii, I., Fukushima, N., Ye, X., et al. Lysophospholipid receptors: Signaling and biology. Annu Rev Biochem 73 321-354 (2004).

5 Contos, J.J.A., and Chun, J. The mouse lpA3/Edg7 lysophosphatidic acid receptor gene: genomic structure, chromosomal localization, and expression pattern. Gene 267 243-253 (2001).

6 An, S., Bleu, T., Hallmark, O.G., et al. Characterization of a novel subtype of human G protein-coupled receptor for lysophosphatidic acid. J Biol Chem 273(14) 7906-7910 (1998).

Noguchi, K., Ishii, S., and Shimizu, T. Identification of p2y9/GPR23 as a novel G protein-coupled receptor for lysophosphatidic acid, structurally distant from the Edg family. J Biol Chem 278(28) 25600-25606 (2003).

An, S., Bleu, T., Hallmark, O.G., et al. Characterization of a novel subtype of human G protein-coupled receptor for lysophosphatidic acid. J Biol Chem 273(14) 7906-7910 (1998).

Chun, J., Goetzl, E.J., Hla, T., et al. International union of pharmacology. XXXIV. Lysophospholipid receptor nomenclature. Pharmacol Rev 54 265-269 (2002).

Fukushima, N., and Chun, J. The LPA receptors. Prostaglandins Other Lipid Mediat 64 21-32 (2001).

Ishii, I., Fukushima, N., Ye, X., et al. Lysophospholipid receptors: Signaling and biology. Annu Rev Biochem 73 321-354 (2004).

Contos, J.J.A., and Chun, J. The mouse lpA3/Edg7 lysophosphatidic acid receptor gene: genomic structure, chromosomal localization, and expression pattern. Gene 267 243-253 (2001).

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