Antigen: human doppel amino acids 112-120 (ATQAANQGE) · Host: rabbit · Application(s): WB and IHC (formalin-fixed paraffin-embedded sections); other applications not tested · Doppel is a homolog of the cellular prion protein. Like prion protein, doppel has two N-linked oligosaccharides, and is presented on the cell surface via a glycosylphosphatidylinositol anchor.¹ Unlike prion protein, it lacks the conformationally plastic and octapeptide repeat domains.² The primary physiological role of doppel protein remains to be determined, but there is some evidence suggesting that cell surface prion protein can antagonize the toxic effect of doppel expressed in the central nervous system.¹ In addition, the protein may play a major role in human male fertility, given its expression on both sertoli cells and spermatozoa.³ Cayman Chemical’s doppel polyclonal antibody can be used for western blot and immunohistochemistry (formalin-fixed paraffin-embedded sections) analysis on samples of human, mouse, or rat origin. The antibody recognizes the nonglycosylated and glycosylated forms of the protein.

1 Silverman, G.L., Qin, K., Moore, R.C., et al. Doppel is an N-glycosylated, glycosylphosphatidylinositol-anchored protein. Expression in testis and ectopic production in the brains of Prnp0/0 mice predisposed to purkinje cell loss. J Biol Chem 275(35) 26834-26841 (2000).

2 Mastrangelo, P., and Westaway, D. The prion gene complex encoding PrP^c and Doppel: Insights from mutational analysis. Gene 275 1-18 (2001).

3 Peoc'h, K., Serres, C., Frobert, Y., et al. The human 'prion-like' protein doppel is expressed in both sertoli cells and spermatozoa. J Biol Chem 277(45) 43071-43078 (2002).

Silverman, G.L., Qin, K., Moore, R.C., et al. Doppel is an N-glycosylated, glycosylphosphatidylinositol-anchored protein. Expression in testis and ectopic production in the brains of Prnp0/0 mice predisposed to purkinje cell loss. J Biol Chem 275(35) 26834-26841 (2000).

Mastrangelo, P., and Westaway, D. The prion gene complex encoding PrP^c and Doppel: Insights from mutational analysis. Gene 275 1-18 (2001).

Peoc'h, K., Serres, C., Frobert, Y., et al. The human 'prion-like' protein doppel is expressed in both sertoli cells and spermatozoa. J Biol Chem 277(45) 43071-43078 (2002).