DMPO Nitrone Adduct Polyclonal Antiserum

Cayman Chemical Item Number 10006170

5,5-Dimethyl-1-Pyrroline-N-Oxide Nitrone Adduct

Description

Antigen: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline N-oxide coupled to ovalbumin · Host: rabbit · Application(s): WB, ICC, and ELISA · Measurement of protein radicals is now possible with the development of the 'spin trap immunoassay' ^1,2 Some proteins with endogenous peroxidase activity are especially susceptible to forming DMPO adducts.¹ Cayman’s DMPO Nitrone Adduct Polyclonal Antiserum is a significant improvement over traditional electron paramagnetic resonance (EPR) detection of protein radical adducts. Compared to traditional techniques, immunodetection of spin trap products allows a higher level of sensitivity and throughput with greatly reduced sample consumption.²

1 Detweiler, C.D., Deterding, L.J., Tomer, K.B., et al. Immunological identification of the heart myoglobin radical formed by hydrogen peroxide. Free Radic Biol Med 33(3) 364-369 (2002).

2 Ramirez, D.C., Chen, Y., and Mason, R.P. Immunochemical detection of hemoglobin-derived radicals formed by reaction with hydrogen peroxide: Involvement of a protein-tyrosyl radical. Free Radic Biol Med 34(7) 830-839 (2003).

Synonyms

5,5-Dimethyl-1-Pyrroline-N-Oxide Nitrone Adduct

Formulation

100 µl of rabbit anti-DMPO nitrone adduct polyclonal antiserum

Stability

2 years

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Specificity

Species independent

Background Reading

Detweiler, C.D., Deterding, L.J., Tomer, K.B., et al. Immunological identification of the heart myoglobin radical formed by hydrogen peroxide. Free Radic Biol Med 33(3) 364-369 (2002).

Bonini, M.G., Siraki, A.G., Atanassov, B.S., et al. Immunolocalization of hypochlorite-induced, catalase-bound free radical formation in mouse hepatocytes. Free Radic Biol Med 42 530-540 (2007).

Ramirez, D.C., Chen, Y., and Mason, R.P. Immunochemical detection of hemoglobin-derived radicals formed by reaction with hydrogen peroxide: Involvement of a protein-tyrosyl radical. Free Radic Biol Med 34(7) 830-839 (2003).

Size

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Subtotal

1 ea

$294.00

$0.00

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$0.00

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DMPO Nitrone Adduct Polyclonal Antiserum