Antigen: human PINK1 amino acids 484-504 (LVRALLQREASKRPSARVAAN) · Host: rabbit · Application(s): WB and IHC (formalin-fixed paraffin-embedded sections); other applications not tested · Phosphatase and Tensin Homolog (PTEN) dephosphorylates lipids such as phosphatidylinositol 3,4,5-triphosphate (PIP₃) and its defects contribute to a variety of human cancers. PTEN induced putative kinase 1 (PINK1) was first identified when studying the tumor-suppressive function of the PTEN signaling pathway and is thus believed to be involved in human cancer pathology.¹ The mRNA of PINK1 is expressed ubiquitously among adult tissues with most abundant expression in the heart, skeletal muscle, and testis.¹ PINK1 is located in mitochondria and its homozygous C-terminus mutation is associated with early onset of Parkinson’s disease.² Cayman’s PINK1 polyclonal antibody recognizes primarily the full length protein at about 66 kDa in human, mouse, and rat tissues. In addition, a truncated form of the protein at about 33 kDa is also detected.³

1 Unoki, M., and Nakamura, Y. Growth-suppressive effects of BPOZ and EGR2, two genes involved in the PTEN signaling pathway. Oncogene 20 4457-4465 (2001).

2 Valente, E.M., Abou-Sleiman, P.M., Caputo, V., et al. Hereditary early-onset Parkinson’s disease caused by mutations in PINK1. Science 304 1158-1160 (2004).

3 Mammalian Gene Collection (MGC) Program Team. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci USA 99(26) 16899-16903 (2002).

Valente, E.M., Abou-Sleiman, P.M., Caputo, V., et al. Hereditary early-onset Parkinson’s disease caused by mutations in PINK1. Science 304 1158-1160 (2004).

Unoki, M., and Nakamura, Y. Growth-suppressive effects of BPOZ and EGR2, two genes involved in the PTEN signaling pathway. Oncogene 20 4457-4465 (2001).

Mammalian Gene Collection (MGC) Program Team. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci USA 99(26) 16899-16903 (2002).