Cayman Chemical | Ghrelin (human acylated) EIA Kit | Description • Ghrelin (human acylated) ELISA Kit

10006306 Ghrelin (human acylated) EIA Kit

Ghrelin (human acylated) ELISA Kit

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Available through Cayman Chemical within America, Asia and Oceania; elsewhere, please contact SPI-Bio <http://www.spibio.com/>

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Limit of detection: 1.5 pg/ml after 20 hour immunological incubation; 4 pg/ml after 3 hour immunological incubation · Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, is synthesized principally in the stomach. It stimulates food intake and transduces signals to hypothalamic regulatory nuclei that control energy homeostasis. The peptide consists of 28 amino acids, with an octanoylation of the serine-3 residue, which is necessary for biological activity. Ghrelin is present in the peripheral circulation in two forms: acylated and non-acylated. This EIA kit specifically measures the acylated form of ghrelin. This EIA is based on a double-antibody sandwich technique. The wells of the plate supplied with the kit are coated with a monoclonal antibody specific to the C-terminal part of ghrelin. This antibody will bind to any ghrelin introduced into the wells (standard or sample). The AChE - Fab' conjugate which recognizes the N-terminal part of acylated ghrelin is also added to the wells. This allows the two antibodies to form a sandwich by binding on different parts of the human acylated ghrelin. Each kit contains ghrelin (acylated) AChE-Fab' conjugate, human acylated ghrelin standard, human acylated ghrelin quality control, Ellman’s reagent, EIA buffer, wash buffer, Tween 20, plates pre-coated with anti-ghrelin mouse monoclonal antibody, and complete instructions.

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	• US Letter paper 8½″ × 11″
	• A4 paper 21cm × 29.7cm

Purchase 10006306 Ghrelin (human acylated) EIA Kit

Pricing is for North America only. Other customers should contact a distributor in their region.

This product is also available to buy in bulk quantities. Please contact our Sales Department for a quote or to purchase.

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Warning This product is not for human or veterinary use.

Pricing updated 2010-03-19.
Prices are subject to change without notice.

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Many of our kits require a source of UltraPure Water. If you do not have a source it is available for purchase from Cayman Chemical. Please check the Additional Items Required section of this kit booklet to verify if Ultra Pure Water is needed. Please note that the 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution. Please see the individual kit booklets for these details or contact techserv@caymanchem.com.