Antigen: synthetic peptide from human HSL amino acids 731-741 (LCRETRQAAEL) · Host: rabbit · Application(s): WB ; other applications not tested · Hormone sensitive lipase (HSL) catalyzes the hydrolysis of tri-, di-, and monoacylglycerols, as well as cholesterol esters and thus mobilizes fatty acid and provides a primary source of energy in mammals.¹ The enzyme is highly expressed in adipose tissue and steroidogenic tissues, and less abundantly in skeletal muscle, heart, brain, pancreatic beta cells, adrenal gland, ovaries, testes, and macrophages. Its presence in various tissues indicates the enzyme plays diverse roles including those in steroidogenesis and spermatogenesis, foam cell formation in atherosclerosis, and diabetic pathology.^1,2 Human HSL cDNA encodes a 775 amino acid protein with an estimated molecular size of 84 kDa.³ A second, larger isoform encoded by a unique testis mRNA was later identified.^4,5 Cayman’s HSL polyclonal antibody detects the enzyme at 86 kDa by western blotting from adipose tissue.

1 Saltiel, A.R. Another hormone-sensitive triglyceride lipase in fat cells. Proc Natl Acad Sci USA 97(2) 535-537 (2000 Jan 18).

2 Kraemer, F.B., and Shen, W. Hormone-sensitive lipase: Control of intracellular tri-(di-)acylglycerol and cholesteryl ester hydrolysis. J Lipid Res 43 1585-1594 (2002 Jan 1).

3 Langin, D., Laurell, H., Holst, L., et al. Gene organization and primary structure of human hormone-sensitive lipase: Possible significane of a sequence homology with a lipase of Moraxella TA144, an antarctic bacterium. Proc Natl Acad Sci USA 90 4897-4901 (2005).

4 Holst, L.S., Langin, D., Mulder, H., et al. Molecular cloning, genomic organization, and expression of a testicular isoform of hormone-sensitive lipase. Genomics 35 441-447 (1996 Jan 1).

5 mairal, A., Melaine, N., Laurell, H., et al. Characterization of a novel testicular form of human hormone-sensitive lipase. Biochem Biophys Res Commun 291 286-290 (2002 Jul 20).

Saltiel, A.R. Another hormone-sensitive triglyceride lipase in fat cells. Proc Natl Acad Sci USA 97(2) 535-537 (2000 Jan 18).

Kraemer, F.B., and Shen, W. Hormone-sensitive lipase: Control of intracellular tri-(di-)acylglycerol and cholesteryl ester hydrolysis. J Lipid Res 43 1585-1594 (2002 Jan 1).

mairal, A., Melaine, N., Laurell, H., et al. Characterization of a novel testicular form of human hormone-sensitive lipase. Biochem Biophys Res Commun 291 286-290 (2002 Jul 20).

Holst, L.S., Langin, D., Mulder, H., et al. Molecular cloning, genomic organization, and expression of a testicular isoform of hormone-sensitive lipase. Genomics 35 441-447 (1996 Jan 1).

Langin, D., Laurell, H., Holst, L., et al. Gene organization and primary structure of human hormone-sensitive lipase: Possible significane of a sequence homology with a lipase of Moraxella TA144, an antarctic bacterium. Proc Natl Acad Sci USA 90 4897-4901 (2005).

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