See personalized New Product recommendations! Get personalized New Product recommendations! Register or Login for personalized New Product recommendations!

Holiday Notification: Cayman Chemical will be closed Monday, May 28, 2012, in observance of the Memorial Day holiday. More…
Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

Hormone Sensitive Lipase Blocking Peptide

Cayman Chemical Item Number 10006372

HSL; LIPE

See more

Description

Peptide sequence: human hormone sensitive lipase (HSL) amino acids 731-741 (LCRETRQAAEL) · To be used in conjunction with Cayman’s HSL polyclonal antibody (Catalog No. 10006371) to block protein-antibody complex formation during immunochemical analysis of HSL · Hormone sensitive lipase (HSL) catalyzes the hydrolysis of tri-, di-, and monoacylglycerols, as well as cholesterol esters and thus mobilizes fatty acid and provides a primary source of energy in mammals.1 The enzyme is highly expressed in adipose tissue and steroidogenic tissues, and less abundantly in skeletal muscle, heart, brain, pancreatic beta cells, adrenal gland, ovaries, testes, and macrophages. Its presence in various tissues indicates the enzyme plays diverse roles including those in steroidogenesis and spermatogenesis, foam cell formation in atherosclerosis, and diabetic pathology.1,2 Human HSL cDNA encodes a 775 amino acid protein with an estimated molecular size of 84 kDa.3 A second, larger isoform encoded by a unique testis mRNA was later identified.4,5 Cayman’s HSL polyclonal antibody detects the enzyme at 86 kDa by western blotting from adipose tissue and mouse macrophage whereas it detects mainly the testicular isoform at approximately 130 kDa from ram seminal vesicle, HEK293 and THP-1 cells.

1 Saltiel, A.R. Another hormone-sensitive triglyceride lipase in fat cells. Proc Natl Acad Sci USA 97(2) 535-537 (2000 Jan 18).

2 Kraemer, F.B., and Shen, W. Hormone-sensitive lipase: Control of intracellular tri-(di-)acylglycerol and cholesteryl ester hydrolysis. J Lipid Res 43 1585-1594 (2002 Jan 1).

3 Langin, D., Laurell, H., Holst, L., et al. Gene organization and primary structure of human hormone-sensitive lipase: Possible significane of a sequence homology with a lipase of Moraxella TA144, an antarctic bacterium. Proc Natl Acad Sci USA 90 4897-4901 (2005).

4 Holst, L.S., Langin, D., Mulder, H., et al. Molecular cloning, genomic organization, and expression of a testicular isoform of hormone-sensitive lipase. Genomics 35 441-447 (1996 Jan 1).

5 mairal, A., Melaine, N., Laurell, H., et al. Characterization of a novel testicular form of human hormone-sensitive lipase. Biochem Biophys Res Commun 291 286-290 (2002 Jul 20).

Synonyms
  • HSL
  • LIPE
Formulation 200 µg of peptide in 200 µl TBS, pH 7.4, containing 0.1% BSA and 0.02% sodium azide
Stability 2 years
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Saltiel, A.R. Another hormone-sensitive triglyceride lipase in fat cells. Proc Natl Acad Sci USA 97(2) 535-537 (2000 Jan 18).

Kraemer, F.B., and Shen, W. Hormone-sensitive lipase: Control of intracellular tri-(di-)acylglycerol and cholesteryl ester hydrolysis. J Lipid Res 43 1585-1594 (2002 Jan 1).

mairal, A., Melaine, N., Laurell, H., et al. Characterization of a novel testicular form of human hormone-sensitive lipase. Biochem Biophys Res Commun 291 286-290 (2002 Jul 20).

Holst, L.S., Langin, D., Mulder, H., et al. Molecular cloning, genomic organization, and expression of a testicular isoform of hormone-sensitive lipase. Genomics 35 441-447 (1996 Jan 1).

Langin, D., Laurell, H., Holst, L., et al. Gene organization and primary structure of human hormone-sensitive lipase: Possible significane of a sequence homology with a lipase of Moraxella TA144, an antarctic bacterium. Proc Natl Acad Sci USA 90 4897-4901 (2005).

Show all 5 Hide all but first 3
Size Price Quantity Subtotal
1 ea $107.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

Related Products

Hormone Sensitive Lipase Polyclonal Antibody

Downloads

Batch-specific Information

Login to access batch-specific information

Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

Other Resources
Management Team Company Profile Company History ChemAssistant Tools FAQs Cayman Europe Cayman Pharma CaBRI Cayman Gear Press Releases Illustrations and Charts Key Research Area Posters Article Library Analysis Tools Conference Schedule Order Terms Browser Recommendation Privacy Statement Site Map

Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640

Copyright 2012 Cayman Chemical Company

Lost password?