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HAT Inhibitor Screening Assay Kit

Cayman Chemical Item Number 10006515

Histone Acetyltransferase Inhibitor Screening Assay Kit

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Description

Cayman’s Histone Acetyltransferase (HAT) Inhibitor Screening Assay Kit provides a fast, fluorescence-based method for evaluating pCAF HAT inhibitors. The procedure requires only three easy steps, all performed in the same microwell plate. In the first step of the protocol, HAT is incubated with acetyl-CoA and the histone H3 peptide. During this time, HAT catalyzes the enzymatic transfer of acetyl groups from acetyl-CoA to the H3 peptide producing an acetylated peptide and CoASH. Following addition of isopropanol to stop the enzymatic reaction, CPM is added to the wells of the plate. CPM reacts with the free thiol groups present on CoASH forming a highly fluorescent product that is detected using excitation and emission wavelengths of 360-390 and 450-470 nm, respectively.
Synonyms
  • Histone Acetyltransferase Inhibitor Screening Assay Kit
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Grunstein, M. Histone acetylation in chromatin structure and transcription. Nature 389 349-352 (1997 Sep 25).

El-Deiry, W.S. The p53 pathway and cancer therapy. Cancer J 11 229-236 (1998).

Clements, A., Rojas, J.R., Trievel, R.C., et al. Crystal structure of the histone acetyltransferase domain of the human PCAF transcriptional regulator bound to coenzyme A. EMBO J 18(13) 3521-3532 (1999).

Trievel, R.C., Li, F., and Marmorstein, R. Application of a fluorescent histone acetyltransferase assay to probe the substrate specificity of the human p300/CBP-associated factor. Anal Biochem 287 319-328 (2000).

Okumura, K., Mendoza, M., Bachoo, R.M., et al. PCAF modulates PTEN activity. J Biol Chem 281 26562-26568 (2006).

Strahl, B.D., and Allis, D. The language of covalent histone modifications. Nature 403 41-45 (2000 Jan 6).

Cheung, W.L., Briggs, D.B., and Allis, C.D. Acetylation and chromosomal functions. Curr Opin Cell Biol 12 326-333 (2000 Jan 1).

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10006515-96WELL
HAT Assay Buffer (5X) 1 ea
HAT Acetyl CoA 1 ea
Histone Acetyltransferase (pCAF) 1 ea
HAT Peptide 1 ea
HAT Stop Reagent 1 ea
HAT Developer 1 ea
96-Well Solid Plate (white)
Size Price Quantity Subtotal
96 wells $300.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample HAT Inhibitor Screening Assay Kit under the Cayman Challenge program.

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