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Source:
recombinant N-terminal hexahistidine-tagged protein expressed in E. coli
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Mr:
24.3 kDa
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Protein concentration:
XX mg/ml
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One unit of enzyme produces one µmole of PGD2/min at 25°C in 100 mM Tris-HCl buffer, pH 8.0, containing 1 mM GSH, 1 mg/ml magnesium chloride, and 40 µM PGH2 · Prostaglandin D synthase (PGD synthase) catalyzes the isomerization of PGH2 to produce PGD2. PGD2 induces sleep, regulates nociception, inhibits platelet aggregation, and acts as an allergic mediator. Two distinct types of PGD synthase have been identified, namely the lipocalin-type enzyme (β-trace) and the hematopoietic enzyme.1,2,3,4 Hematopoietic PGD synthase (H-PGDS) is widely distributed in the peripheral tissues and is localized in the antigen-presenting cells, mast cells, and megakaryocytes. PGD2 is produced by H-PGDS in large quantities by allergen-stimulated mast cells and acts as a pro-inflammatory mediator in allergic reactions. H-PGDS requires glutathione for activity and belongs to the sigma-class of glutathione-S-transferases.1,4,5 Cayman Chemical’s H-PGDS is a highly-purified, active recombinant enzyme isolated from an E. Coli expression system. The enzyme is ideally suited for a variety of biochemical experiments including mechanistic studies and development of selective enzyme inhibitors.
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1
Kanaoka, Y., Fujimori, K., Kikuno, R., et al. Structure and chromosomal localization of human and mouse genes for hematopoietic prostaglandin D synthase. Eur J Biochem 267 3315-3322 (2000).
2
Jowsey, I.R., Thomson, A.M., Flanagan, J.U., et al. Mammalian class sigma glutathione s-transferases: Catalytic properties and tissue-specific expression of human and rat GSH-dependent prostaglandin D2 synthases. Biochem J 359 507-516 (2001).
3
Urade, Y., Watanabe, K., Hayaishi, O. Prostaglandin D, E, and F synthases. J Lipid Mediat Cell Signal 12 257-273 (1995).
4
Kanaoka, Y., Ago, H., Inagaki, E., et al. Cloning and crystal structure of hematopoietic prostaglandin D synthase. Cell 90 1085-1095 (1997).
5
Urade, Y., Fujimoto, N., Ujihara, M., et al. Biochemical and immunological characterization of rat spleen prostaglandin D synthetase. J Biol Chem 262(8) 3820-3825 (1987).
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