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14,15-Leukotriene C4 EIA Kit

Cayman Chemical Item Number 10006748

14,15-LTC4 EIA Kit; 14,15-Leukotriene C4 ELISA Kit

14,15-Leukotriene C4 EIA Kit

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14,15-Leukotriene C4 EIA Kit Analysis Tools

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Description

Limit of detection: 80% B/B0: 18 pg/ml · Sensitivity: 50% B/B0: 66 pg/ml · The leukotrienes (LTs) were discovered in 1979 as a group of acute inflammatory mediators derived from arachidonic acid in leukocytes. Although the majority of leukotrienes are formed through the 5-lipoxygenase (5-LO) pathway, a second family of leukotrienes can be formed through an alternate pathway involving the dual actions of 15- and 12-LOs.1,2,3,4 The resulting epoxytriene, 14,15-LTA4, can be converted to 14,15-LTC4, as demonstrated in sheep uterus and RBL-1 cells. Although many of the physiological actions of 14,15-LTC4 remain to be elucidated, it has been shown to have weak contractile activity on both guinea pig ileum and pulmonary parenchyma.5

1 Yokoyama, C., Shinjo, F., Yoshimoto, T., et al. Arachidonate 12-lipoxygenase purified from porcine leukocytes by immunoaffinity chromatography and its reactivity with hydroperoxyeicosatetraenoic acids. J Biol Chem 261 16714-16721 (1986).

2 Bryant, R.W., Schewe, T., Rapoport, S.M., et al. Leukotriene formation by a purified reticulocyte lipoxygenase enzyme. Conversion of arachidonic acid and 15-hydroperoxyeicosatetraenoic acid to 14,15-leukotriene A4. J Biol Chem 260 3548-3555 (1985).

3 Sok, D., Han, C., Shieh, W., et al. Enzymatic formation of 14,15-leukotriene A and C(14)-sulfur-linked peptides. Biochem Biophys Res Commun 104(4) 1363-1370 (1982).

4 Sailesh, S., Kumar, Y.V.K., Prasad, M., et al. Sheep uterus dual lipoxygenase in the synthesis of 14,15-leukotrienes. Arch Biochem Biophys 315(2) 362-368 (1994).

5 Drazen, J.M., Lewis, R.A., Austen, K.F., et al. Contractile activities of structural analogs of leukotrienes C and D: Necessity of a hydrophobic region. Proc Natl Acad Sci USA 78(5) 3195-3198 (1987).

Synonyms
  • 14,15-LTC4 EIA Kit
  • 14,15-Leukotriene C4 ELISA Kit
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere
Specificity
14,15-Leukotriene C4 100%
14,15-Leukotriene D4 0.90%
14,15-Leukotriene E4 0.04%
Leukotriene C4 <0.01%
Leukotriene D4 <0.01%
Leukotriene E4 <0.01%
Arachidonic Acid <0.01%
(±)5-HETE <0.01%
(±)8-HETE <0.01%
(±)9-HETE <0.01%
(±)11-HETE <0.01%
(±)12-HETE <0.01%
(±)15-HETE <0.01%
5(S),6(S)-diHETE <0.01%
5(S),15(S)-diHETE <0.01%
8(S),15(S)-diHETE <0.01%
14,15-diHETrE <0.01%
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Background Reading

Yokoyama, C., Shinjo, F., Yoshimoto, T., et al. Arachidonate 12-lipoxygenase purified from porcine leukocytes by immunoaffinity chromatography and its reactivity with hydroperoxyeicosatetraenoic acids. J Biol Chem 261 16714-16721 (1986).

Bryant, R.W., Schewe, T., Rapoport, S.M., et al. Leukotriene formation by a purified reticulocyte lipoxygenase enzyme. Conversion of arachidonic acid and 15-hydroperoxyeicosatetraenoic acid to 14,15-leukotriene A4. J Biol Chem 260 3548-3555 (1985).

Sok, D., Han, C., Shieh, W., et al. Enzymatic formation of 14,15-leukotriene A and C(14)-sulfur-linked peptides. Biochem Biophys Res Commun 104(4) 1363-1370 (1982).

Sailesh, S., Kumar, Y.V.K., Prasad, M., et al. Sheep uterus dual lipoxygenase in the synthesis of 14,15-leukotrienes. Arch Biochem Biophys 315(2) 362-368 (1994).

Drazen, J.M., Lewis, R.A., Austen, K.F., et al. Contractile activities of structural analogs of leukotrienes C and D: Necessity of a hydrophobic region. Proc Natl Acad Sci USA 78(5) 3195-3198 (1987).

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10006748-96WELL
14,15-Leukotriene C4 AChE Tracer 100 dtn
14,15-Leukotriene C4 EIA Antiserum 100 dtn
14,15-Leukotriene C4 EIA Standard 1 ea
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
10006748-96SOLID
14,15-Leukotriene C4 AChE Tracer 100 dtn
14,15-Leukotriene C4 EIA Antiserum 100 dtn
14,15-Leukotriene C4 EIA Standard 1 ea
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Solid Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
10006748-480WELL
14,15-Leukotriene C4 AChE Tracer 500 dtn
14,15-Leukotriene C4 EIA Antiserum 500 dtn
14,15-Leukotriene C4 EIA Standard 1 ea
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
10006748-480SOLID
14,15-Leukotriene C4 AChE Tracer 500 dtn
14,15-Leukotriene C4 EIA Antiserum 500 dtn
14,15-Leukotriene C4 EIA Standard 1 ea
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Solid Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Size Price Quantity Subtotal
96 solid wells $320.00 $0.00
96 strip wells $320.00 $0.00
480 solid wells $1,200.00 $0.00
480 strip wells $1,200.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample 14,15-Leukotriene C4 EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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