Antigen: human sphingosine kinase 1 (SPHK 1) amino acids 264-274 (DLESEKYRRLG) · Host: rabbit · Application(s): WB and ICC; other applications not tested · SPHK 1 catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate. This reaction plays an important role in determining cell proliferation versus cell death.^1,2 SPHK 1 is found in a wide variety of tissues and cell types including kidney, liver, spleen, heart, platelets, and human tumors. On a cellular level, it is found in the cytosolic and membrane fractions.³ Based on the amino acid sequence, this protein has a molecular weight of approximately 43 kDa. The observed band at 50 kDa may be explained by some reported post translational modifications.⁴

1 Delon, C., Manifava, M., Wood, E., et al. Sphingosine kinase 1 is an intracellular effector of phosphatidic acid. J Biol Chem 279(43) 44763-44774 (2004).

2 Wang, E., Norred, W.P., Bacon, C.W., et al. Inhibition of sphingolipid biosynthesis by fumonisins. Implications for diseases associated with Fusarium moniliforme. J Biol Chem 266 14486-14490 (1991).

3 Maceyka, M., Sankala, H., Hait, N.C., et al. SphK1 and SphK 2, sphingosine kinase isoenzymes with opposing functions in sphingolipid metabolism. J Biol Chem 280(44) 37118-37129 (2005).

4 Pitson, S.M., D'Andrea, R.J., Vandeleur, L., et al. Human sphingosine kinase: Purification, molecular cloning and characterization of the native and recombinant enzymes. Biochem J 350 429-441 (2000).

Delon, C., Manifava, M., Wood, E., et al. Sphingosine kinase 1 is an intracellular effector of phosphatidic acid. J Biol Chem 279(43) 44763-44774 (2004).

Maceyka, M., Sankala, H., Hait, N.C., et al. SphK1 and SphK 2, sphingosine kinase isoenzymes with opposing functions in sphingolipid metabolism. J Biol Chem 280(44) 37118-37129 (2005).

Pitson, S.M., D'Andrea, R.J., Vandeleur, L., et al. Human sphingosine kinase: Purification, molecular cloning and characterization of the native and recombinant enzymes. Biochem J 350 429-441 (2000).

Wang, E., Norred, W.P., Bacon, C.W., et al. Inhibition of sphingolipid biosynthesis by fumonisins. Implications for diseases associated with Fusarium moniliforme. J Biol Chem 266 14486-14490 (1991).

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