Renin Fluorogenic Substrate
Cayman Chemical Item Number 10006906
Description
Renin is an aspartyl protease of approximately 40 kDa that converts angiotensinogen into angiotensin I.1 The renin fluorogenic substrate consists of the normal peptide substrate for renin which has been linked to the fluorophore EDANS at one end and to a non-fluorescent quenching molecule (Dabcyl) at the other.2 After cleavage by renin, the product (peptide-EDANS) is brightly fluorescent and can be easily analyzed using an excitation wavelength of 340 nm and emission wavelengths of 485-510 nm.
1
Imai, T., Miyazaki, H., Hirose, S., et al. Cloning and sequence analysis of cDNA for human renin precursor. Proc Natl Acad Sci USA 80 7405-7409 (1983).
2
Wang, G.T., Chung, C.C., Holzman, T.F., et al. A continuous fluorescence assay of renin activity. Anal Biochem 210 351-359 (1993).
| Formal Name |
Arg-Glu(EDANS)-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys(Dabcyl)-Arg-OH |
| Formula Weight |
2282.7 |
| Formulation |
A red/brown crystalline solid |
| Purity |
>95% |
| Stability |
1 year |
| Storage |
-20°C |
| Shipping |
Wet ice
in continental US; may vary elsewhere
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Background Reading
Imai, T., Miyazaki, H., Hirose, S., et al. Cloning and sequence analysis of cDNA for human renin precursor. Proc Natl Acad Sci USA 80 7405-7409 (1983).
Wang, G.T., Chung, C.C., Holzman, T.F., et al. A continuous fluorescence assay of renin activity. Anal Biochem 210 351-359 (1993).
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Pricing updated 2012-02-12.
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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.
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