Adipogenesis Assay Kit

Cayman Chemical Item Number 10006908

Adipogenesis Assay Kit

Description

Obesity is a growing concern worldwide and has reached epidemic proportions in the United States.¹ It is a risk factor in many major chronic diseases that afflict our society, including cardiovascular disease, diabetes mellitus, and cancer. In recent years, numerous studies have focused on identifying the mechanism of development of obesity, which is a process of either increasing the number of fat cells (fat cell hyperplasia) or enlargement of fat cells with each cell carrying greater amounts of fat (fat cell hypertrophy), or both.^2,1 The ability to regulate the cell cycle and differentiation of adipocytes are key in the development and physiology of obesity and also in the origin of cancer. Understanding of these processes is critical to a rational approach to the treatment of obesity and cancer. Cayman’s Adipogenesis Assay Kit provides the reagents required for studying the induction and inhibition of adipogenesis in the established 3T3-L1 model, using the adipogenesis induction procedure. This kit can also be used to screen drug candidates involved in adipogenesis. The classic Oil Red O staining for lipid droplets is used in this kit as an indicator of the degree of adipogenesis, and can be quantified with a plate reader after the dye is conveniently extracted from the lipid droplet.

1 Camp, H.S., Ren, D., and Leff, T. Adipogenesis and fat-cell function in obesity and diabetes. Trends Mol Med 8(9) 442-447 (2002).

2 Spiegelman, B.M., and Flier, J.S. Adipogenesis and obesity: Rounding out the big picture. Cell 87 377-389 (1995).

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Background Reading

Camp, H.S., Ren, D., and Leff, T. Adipogenesis and fat-cell function in obesity and diabetes. Trends Mol Med 8(9) 442-447 (2002).

Spiegelman, B.M., and Flier, J.S. Adipogenesis and obesity: Rounding out the big picture. Cell 87 377-389 (1995).

Tong, Q., and Hotamisligil, G.S. Molecular mechanisms of adipocyte differentation. Rev Endocr Metab Disord 2 349-355 (2001).

Prusty, D., Park, B., Davis, K.E., et al. Activation of MEK/ERK signaling promotes adipogenesis by enhancing peroxisome proliferator-activated receptor γ (PPARγ) and C/EBPα gene expression during the differentiation of 3T3-L1 preadipocytes. J Biol Chem 277(48) 46226-46232 (2002).

Scherer, P.E., Lisanti, M.P., Baldini, G., et al. Induction of caveolin during adipogenesis and association of GLUT4 with caveolin-rich vesicles. J Cell Biol 127 1233-1243 (1994).

Thompson, G.M., Trainor, D., Biswas, C., et al. A high-capacity assay for PPARγ ligand regulation of endogenous aP2 expression in 3T3-L1 cells. Anal Biochem 330 21-28 (2004).

Vidal-Puig, A., Jimenez-Linan, M., Lowell, B.B., et al. Regulation of PPAR γ gene expression by nutrition and obesity in rodents. J Clin Invest 97 2553-2561 (1996).

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10006908-1EA

Cell-Based Assay IBMX Solution (1,000X)

1 ea

Adipogenesis Assay Insulin Solution (1,000X)

1 ea

Adipogenesis Assay Dexamethasone Solution (1,000X)

1 ea

Lipid Droplets Assay Fixative (10X)

1 ea

Lipid Droplets Assay Wash Solution

2 × 1 ea