See personalized New Product recommendations! Get personalized New Product recommendations! Register or Login for personalized New Product recommendations!

Holiday Notification: Cayman Chemical will be closed Monday, May 28, 2012, in observance of the Memorial Day holiday. More…
Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

ChREBP Transcription Factor Assay Kit

Cayman Chemical Item Number 10006909

Carbohydrate Response Element-Binding Protein

ChREBP Transcription Factor Assay Kit

See more

Description

Cayman’s ChREBP Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the ChREBP response element is immobilized onto the wells of a 96 well plate. ChREBP contained in a nuclear extract binds specifically to the ChREBP response element. ChREBP is detected by addition of specific primary antibody directed against ChREBP. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.
Synonyms
  • Carbohydrate Response Element-Binding Protein
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Ishii, S., Iizuka, K., Miller, B.C., et al. Carbohydrate response element binding protein directly promotes lipogenic enzyme gene transcription. Proc Natl Acad Sci USA 101(44) 15597-15602 (2004).

Uyeda, K., Yamashita, H., and Kawaguchi, T. Carbohydrate responsive element-binding protein (ChREBP): A key regulator of glucose metabolism and fat storage. Biochem Pharmacol 63 2075-2080 (2002).

Iizuka, K., Miller, B., and Uyeda, K. Deficiency of a carbohydrate-activated transcription factor, ChREBP, prevents obesity and improves plasma glucose control in leptin deficient (ob/ob) mice. Am J Physiol Endocrinol Metab 291 E358-E364 (2006).

10006909-96WELL
Transcription Factor Binding Assay Buffer (4X) 1 ea
Transcription Factor Antibody Binding Buffer (10X) 1 ea
Transcription Factor Goat Anti-Rabbit HRP Conjugate 1 ea
Transcription Factor Developing Solution 1 ea
Transcription Factor Stop Solution 1 ea
Transcription Factor Reagent A 1 ea
Transcription Factor ChREBP Positive Control 1 ea
Transcription Factor ChREBP 96-Well Strip Plate 1 ea
Transcription Factor ChREBP Primary Antibody 1 ea
Transcription Factor ChREBP Competitor dsDNA 1 ea
Polysorbate 20 1 ea
Wash Buffer Concentrate (400X) 5 mL
Size Price Quantity Subtotal
96 wells $410.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample ChREBP Transcription Factor Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Related Products

ATF2 (Phospho-Thr69,71) Transcription Factor Assay Kit
ChREBP Blocking Peptide
ChREBP Cell-Based Translocation Assay Kit
ChREBP DBD (human recombinant)
Coenzyme A Assay Kit
Creatine Kinase Fluorometric Assay Kit New
CREB (Phospho-Ser133) Transcription Factor Assay Kit
Fructose Fluorometric Assay Kit New
Glucose Colorimetric Assay Kit
Glucose Fluorometric Assay Kit New
Glycerol Colorimetric Assay Kit
Glycerol Fluorometric Assay Kit New
HIF-1α Transcription Factor Assay Kit
NF-κB (human p50/p65) Combo Transcription Factor Assay Kit
NF-κB (human p50) Transcription Factor Assay Kit
NF-κB (p50) (human recombinant)
NF-κB (p65) Transcription Factor Assay Kit
Nonidet P-40 Assay Reagent (10%)
Nuclear Extraction Kit
p53 Designer Transcription Factor Assay Kit
p53 Transcription Factor Assay Kit
PPARα Transcription Factor Assay Kit
PPARα, δ, γ Complete Transcription Factor Assay Kit
PPARγ Transcription Factor Assay Kit
PPARδ Transcription Factor Assay Kit
SREBP-1 Transcription Factor Assay Kit
SREBP-2 Transcription Factor Assay Kit
Triglyceride Fluorometric Assay Kit New
Show all 28 Hide all but first 3

Downloads

Batch-specific Information

Login to access batch-specific information

Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

Other Resources
Management Team Company Profile Company History ChemAssistant Tools FAQs Cayman Europe Cayman Pharma CaBRI Cayman Gear Press Releases Illustrations and Charts Key Research Area Posters Article Library Analysis Tools Conference Schedule Order Terms Browser Recommendation Privacy Statement Site Map

Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640

Copyright 2012 Cayman Chemical Company

Lost password?