See personalized New Product recommendations! Get personalized New Product recommendations! Register or Login for personalized New Product recommendations!

Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

PPARδ Transcription Factor Assay Kit

Cayman Chemical Item Number 10006914

Peroxisome Proliferator-activated Receptor δ Transcription Factor Assay Kit

PPARδ Transcription Factor Assay Kit

See more

Description

Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors belonging to the large family of nuclear hormone receptors. There are three subtypes, PPARα, β/δ, and γ, which are expressed at variable levels in different tissues.1 Cayman’s PPARδ Transcription Factor Assay is a non-radioactive, sensitive method for detecting PPARδ binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the wells of a 96-well plate. PPARs contained in a nuclear extract bind to the PPRE from which PPARδ is selectively detected using a specific primary antibody. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.

1 Olefsky, J.M. Nuclear receptor minireview series. J Biol Chem 276(40) 36863-36864 (2001).

Synonyms
  • Peroxisome Proliferator-activated Receptor δ Transcription Factor Assay Kit
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Willson, T.M., Brown, P.J., Sternbach, D.D., et al. The PPARs: From orphan receptors to drug discovery. J Med Chem 43(4) 528-550 (2000).

Wang, Y., Lee, C., Tiep, S., et al. Peroxisome-proliferator-activated receptor δ activates fat metabolism to prevent obesity. Cell 113 159-170 (2003).

Gervois, P., Torra, I.P., Fruchart, J., et al. Regulation of lipid and lipoprotein metabolism by PPAR activators. Clin Chem Lab Med 38(1) 3-11 (2000).

Olefsky, J.M. Nuclear receptor minireview series. J Biol Chem 276(40) 36863-36864 (2001).

Smith, S.A. Peroxisomal proliferater-activated receptors and the regulation of lipid oxidation and adipogenesis. Biochem Soc Trans 25 1242-1248 (1997).

Chawla, A., Repa, J.J., Evans, R.M., et al. Nuclear receptors and lipid physiology: Opening the x-files. Science 294 1866-1870 (2001).

Show all 6 Hide all but first 3
10006914-96WELL
Transcription Factor Binding Assay Buffer (4X) 1 ea
Transcription Factor Antibody Binding Buffer (10X) 1 ea
Transcription Factor Goat Anti-Rabbit HRP Conjugate 1 ea
Transcription Factor PPAR Competitor dsDNA 1 ea
Transcription Factor PPAR 96-Well Strip Plate 1 ea
Transcription Factor Developing Solution 1 ea
Transcription Factor Stop Solution 1 ea
Transcription Factor PPARδ Positive Control 1 ea
Transcription Factor PPARδ Primary Antibody 1 ea
Transcription Factor Reagent A 1 ea
Polysorbate 20 1 ea
Wash Buffer Concentrate (400X) 5 mL
Size Price Quantity Subtotal
96 wells $410.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample PPARδ Transcription Factor Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Related Products

ChREBP Transcription Factor Assay Kit
Coenzyme A Assay Kit
CREB (Phospho-Ser133) Transcription Factor Assay Kit
GW 0742
HIF-1α Transcription Factor Assay Kit
NF-κB (human p50/p65) Combo Transcription Factor Assay Kit
NF-κB (p65) Transcription Factor Assay Kit
Nuclear Extraction Kit
PPARα Polyclonal Antibody
PPARα Transcription Factor Assay Kit
PPARα, δ, γ Complete Transcription Factor Assay Kit
PPARγ Polyclonal Antibody
PPARγ Transcription Factor Assay Kit
PPARδ (human recombinant)
PPARδ Polyclonal Antibody
Serum Retinol Binding Protein 4 Polyclonal Antibody Exclusive
Show all 16 Hide all but first 3

Downloads

Batch-specific Information

Login to access batch-specific information

Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

Other Resources
Management Team Company Profile Company History ChemAssistant Tools FAQs Cayman Europe Cayman Pharma CaBRI Cayman Gear Press Releases Illustrations and Charts Key Research Area Posters Article Library Analysis Tools Conference Schedule Order Terms Browser Recommendation Privacy Statement Site Map

Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640

Copyright 2012 Cayman Chemical Company

Lost password?