Cayman Chemical | PPARα Transcription Factor Assay Kit | Description • Peroxisome Proliferator-activated Receptor α Transcription Factor Assay Kit

10006915 PPARα Transcription Factor Assay Kit

Peroxisome Proliferator-activated Receptor α Transcription Factor Assay Kit

• See more Transcription Factor Assay; Assay Kits; Nuclear Receptors; PPAR; Atherosclerosis; Endocrinology; Metabolic Syndrome; Metabolism; Obesity

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Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors belonging to the large family of nuclear hormone receptors. There are three subtypes, PPARα, β/δ, and γ, which are expressed at variable levels in different tissues.¹ The Cayman Chemical PPARα Transcription Factor Assay is a non-radioactive, sensitive method for detecting PPARα binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double-stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the wells of a 96-well plate. PPARs contained in a nuclear extract bind to the PPRE and PPARα is selectively detected using a specific primary antibody. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.

1 Olefsky, J.M. Nuclear receptor minireview series. J Biol Chem 276(40) 36863-36864 (2001).

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	Download Material Safety Data Sheet (MSDS) 205 Kb PDF
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Purchase 10006915 PPARα Transcription Factor Assay Kit

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Pricing updated 2010-03-17.
Prices are subject to change without notice.