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Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

Luminex® Cysteinyl Leukotriene Kit

Cayman Chemical Item Number 10007577

Luminex® Cysteinyl Leukotriene Kit

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Description

Limit of detection: 80% B/B0: 15 pg/ml · Sensitivity: 50% B/B0: 90 pg/ml · Cayman’s Luminex® CysLT Kit is the first of its kind for the measurement of CysLTs using Luminex® xMAP® technology. For application of this technology to the measurement of CysLT in a competitive format, microspheres have been coated with Cayman’s cysLT monoclonal antibody. The assay is based on the competition between CysLT and a CysLT-phycoerytherin conjugate (CysLT tracer) for the monoclonal antibody binding sites on the microsphere beads. After sufficient equilibration time the assays are read using a Luminex® 100TM or 200TM analyzer.
Stability 6 months
Storage -80°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Leukotriene C4 100%
Leukotriene D4 93%
Leukotriene E4 23.1%
Leuktoriene B4 0.12%
Arachidonic Acid <0.01%
L-cysteine <0.01%
Glutathione <0.01%
Leukotriene C4 methyl ester <0.01%
Prostaglandin D2 <0.01%
Prostaglandin E2 <0.01%
Prostaglandin F2α <0.01%
6-keto Prostaglandin F2α <0.01%
Thromboxane B2 <0.01%
11-dehydro Thromboxane B2 <0.01%
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Background Reading

Samuelsson, B., Dahlén, S., Lindgren JÅ, et al. Leukotrienes and lipoxins: Structures, biosynthesis, and biological effects. Science 237 1171-1176 (1987).

Westcott, J.Y., Johnston, K., Batt, R.A., et al. Measurement of peptidoleukotrienes in biological fluids. Am J Physiol 232 2640-2648 (1990).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Dahlén, S., Björk, J., Hedqvist, P., et al. Leukotrienes promote plasma leakage and leukocyte adhesion in postcapillary venules: In vivo effects with relevance to the acute inflammatory response. Proc Natl Acad Sci USA 78 3887-3891 (1981).

Bihari, D., Smithies, M., Gimson, A., et al. The effects of vasodilation with prostacyclin on oxygen delivery and uptake in critically ill patients. N Engl J Med 317 397-404 (1987).

Campbell, B.J., Baker, S.F., Shukla, S.D., et al. Bioconversion of leukotriene D4 by lung dipeptidase. Biochim Biophys Acta 1042 107-112 (1990).

Samuelsson, B. Leukotrienes: Mediators of immediate hypersensitivity reactions and inflammation. Science 220 568-575 (1983).

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10007577-1EA
Luminex® Assay Buffer Concentrate (10X) 10 mL
Cysteinyl Leukotriene xMAP® Beads 1 ea
Cysteinyl Leukotriene Phycoerythrin Tracer 1 ea
Cysteinyl Leukotriene Luminex® Standard 1 ea
High-Binding 96-Well Strip Plate
Foil Plate Covers
Size Price Quantity Subtotal
1 ea $348.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Luminex® Cysteinyl Leukotriene Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Is this kit right for my sample type? Cysteinyl Leukotriene Recommendation Chart

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Luminex® Prostaglandin E2/Interleukin-1β Duplex Kit
Luminex® Prostaglandin E2 Kit
UltraPure Water
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