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Prorenin (human recombinant)

Cayman Chemical Item Number 10007599

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Description

Source: recombinant enzyme expressed in HEK cells · Mr: 45 kDa · Prorenin is the inactive precursor of renin, which is a key enzyme in the regulation of blood pressure and electrolyte balance. In renal juxtaglomerular cells, sequential cleavage of the N-terminal 20 and 46 amino acids of preprorenin produces prorenin and active renin, respectively.1 Renin catalyzes the conversion of angiotensinogen to angiotensin I.2 Prorenin exhibits 5-10% of the proteolytic activity of renin, however, the blood circulating levels are 10 times higher.3,4

1 Holzman, T.F., Chung, C.C., Edalji, R., et al. Recombinant human prorenin from CHO cells: Expression and purification. J Protein Chem 9(6) 663-672 (1990).

2 Imai, T., Miyazaki, H., Hirose, S., et al. Cloning and sequence analysis of cDNA for human renin precursor. Proc Natl Acad Sci USA 80 7405-7409 (1983).

3 Sealey, J.E., Atlas, S.A., and Laragh, J.H. Prorenin and other large molecular weight forms of renin. Endocr Rev 1(4) 365-391 (1980).

4 Heinrikson, R.L., Hui, J., Zürcher-Neely, H., et al. A structural model to explain the partial catalytic activity of human prorenin. Am J Hypertens 2 367-380 (1989).

Formulation A solution in 50 mM Tris, pH 8.0
Purity ≥99% by SDS-PAGE
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Holzman, T.F., Chung, C.C., Edalji, R., et al. Recombinant human prorenin from CHO cells: Expression and purification. J Protein Chem 9(6) 663-672 (1990).

Heinrikson, R.L., Hui, J., Zürcher-Neely, H., et al. A structural model to explain the partial catalytic activity of human prorenin. Am J Hypertens 2 367-380 (1989).

Imai, T., Miyazaki, H., Hirose, S., et al. Cloning and sequence analysis of cDNA for human renin precursor. Proc Natl Acad Sci USA 80 7405-7409 (1983).

Sealey, J.E., Atlas, S.A., and Laragh, J.H. Prorenin and other large molecular weight forms of renin. Endocr Rev 1(4) 365-391 (1980).

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Size Price Quantity Subtotal
25 µg $214.00 $0.00
50 µg $407.00 $0.00
100 µg $770.00 $0.00
500 µg $3,424.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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Renin (human recombinant)
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