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Cholesterol Assay KitCayman Chemical Item Number 10007640 
Cholesterol Assay Kit
Description
Cholesterol, particularly in the form of low density lipoproteins (LDL), is well understood to be associated with increased risk of coronary heart disease. The measurement of cholesterol is one of the most common tests performed in the clinical laboratory setting. However, simple and easy assays for cholesterol in the research lab have not been readily available. Cayman’s Cholesterol Assay provides a simple fluorometric method for the sensitive quantitation of total cholesterol in plasma or serum. In our assay, cholesteryl esters in the sample are first hydrolyzed by cholesterol esterase. All cholesterol, whether previously esterified or existing free in the circulation, is then oxidized by cholesterol oxidase to the corresponding ketone and hydrogen peroxide. ADHP (10-
Background ReadingMatsuura, E., and Lopez, L.R. Are oxidized LDL/β2- Linsel-Nitschke, P., and Tall, A.R. HDL as a target in the treatment of atherosclerotic cardiovascular disease. Nat Rev Drug Discov 4 193-205 (2005). Kishi, K., Ochiai, K., Ohta, Y., et al. Highly sensitive cholesterol assay with enzymatic cycling applied to measurement of remnant lipoprotein- Li, D., Sinclair, A., Mann, N., et al. The association of diet and thrombotic risk factors in healthy male vegetarians and meat- Jauhiainen, M., and Dolphin, P.J. Human plasma lecithin- Ledwozyw, A., Michalak, J., Stepien, A., et al. The relationship between plasma triglycerides, cholesterol, total lipids, and lipid peroxidation products during human atherosclerosis. Clin Chim Acta 155 275-284 (1986). McNamara, D.J., Kolb, R., Parker, T.S., et al. Heterogeneity of cholesterol homeostasis in man. Response to changes in dietary fat quantity and cholesterol quanity. J Clin Invest 79 1729-1739 (1987). Amundson, D.M., and Zhou, M. Fluorometric method for the enzymatic determination of cholesterol. J Biochem Biophys Meth 38 43-52 (1999). Show all 8 Hide all but first 3 |
This product is also available to buy in bulk quantities. Please contact our Sales Department for a quote or to purchase. Pricing updated 2012-02-10. Prices are subject to change without notice. To ask for assistance with one of our products please contact a Technical Support Scientist. Warning This product is not for human or veterinary use. You may be eligible to receive a free sample Cholesterol Assay Kit under the Cayman Challenge program. Let Cayman analyze your samples for you. See EIA Service for details and availability. Related ProductsShow all 31 Hide all but first 3Downloads
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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins. |
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Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA
Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640
Copyright 2012 Cayman Chemical Company
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